Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoit...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
AOSIS
2009-09-01
|
Series: | Onderstepoort Journal of Veterinary Research |
Online Access: | https://ojvr.org/index.php/ojvr/article/view/40 |
id |
doaj-feb4090d02b044a8890acb04e7e5386e |
---|---|
record_format |
Article |
spelling |
doaj-feb4090d02b044a8890acb04e7e5386e2020-11-24T22:53:46ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352009-09-0176216116510.4102/ojvr.v76i2.4031Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23T. InpankaewS. JittapalapongJ. PhasukN. PinyopanuwutW. ChimnoiC. KengradomkitC SunantaG ZhangG.O. AbogeY. NishikawaI. IgarashiX. XuanCryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4 %, and the seropositive rates for the three provinces were 3.3 % in Chiang Mai, 5.1 % in Chiang Rai and 3 % in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand.https://ojvr.org/index.php/ojvr/article/view/40 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
T. Inpankaew S. Jittapalapong J. Phasuk N. Pinyopanuwut W. Chimnoi C. Kengradomkit C Sunanta G Zhang G.O. Aboge Y. Nishikawa I. Igarashi X. Xuan |
spellingShingle |
T. Inpankaew S. Jittapalapong J. Phasuk N. Pinyopanuwut W. Chimnoi C. Kengradomkit C Sunanta G Zhang G.O. Aboge Y. Nishikawa I. Igarashi X. Xuan Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 Onderstepoort Journal of Veterinary Research |
author_facet |
T. Inpankaew S. Jittapalapong J. Phasuk N. Pinyopanuwut W. Chimnoi C. Kengradomkit C Sunanta G Zhang G.O. Aboge Y. Nishikawa I. Igarashi X. Xuan |
author_sort |
T. Inpankaew |
title |
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 |
title_short |
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 |
title_full |
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 |
title_fullStr |
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 |
title_full_unstemmed |
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 |
title_sort |
seroprevalence of <i>cryptosporidium parvum</i> infection of dairy cows in three northern provinces of thailand determined by enzyme-linked immunosorbent assay using recombinant antigen cpp23 |
publisher |
AOSIS |
series |
Onderstepoort Journal of Veterinary Research |
issn |
0030-2465 2219-0635 |
publishDate |
2009-09-01 |
description |
Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4 %, and the seropositive rates for the three provinces were 3.3 % in Chiang Mai, 5.1 % in Chiang Rai and 3 % in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand. |
url |
https://ojvr.org/index.php/ojvr/article/view/40 |
work_keys_str_mv |
AT tinpankaew seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT sjittapalapong seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT jphasuk seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT npinyopanuwut seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT wchimnoi seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT ckengradomkit seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT csunanta seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT gzhang seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT goaboge seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT ynishikawa seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT iigarashi seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 AT xxuan seroprevalenceoficryptosporidiumparvumiinfectionofdairycowsinthreenorthernprovincesofthailanddeterminedbyenzymelinkedimmunosorbentassayusingrecombinantantigencpp23 |
_version_ |
1725661859818766336 |