Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23

Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoit...

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Main Authors: T. Inpankaew, S. Jittapalapong, J. Phasuk, N. Pinyopanuwut, W. Chimnoi, C. Kengradomkit, C Sunanta, G Zhang, G.O. Aboge, Y. Nishikawa, I. Igarashi, X. Xuan
Format: Article
Language:English
Published: AOSIS 2009-09-01
Series:Onderstepoort Journal of Veterinary Research
Online Access:https://ojvr.org/index.php/ojvr/article/view/40
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spelling doaj-feb4090d02b044a8890acb04e7e5386e2020-11-24T22:53:46ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352009-09-0176216116510.4102/ojvr.v76i2.4031Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23T. InpankaewS. JittapalapongJ. PhasukN. PinyopanuwutW. ChimnoiC. KengradomkitC SunantaG ZhangG.O. AbogeY. NishikawaI. IgarashiX. XuanCryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4 %, and the seropositive rates for the three provinces were 3.3 % in Chiang Mai, 5.1 % in Chiang Rai and 3 % in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand.https://ojvr.org/index.php/ojvr/article/view/40
collection DOAJ
language English
format Article
sources DOAJ
author T. Inpankaew
S. Jittapalapong
J. Phasuk
N. Pinyopanuwut
W. Chimnoi
C. Kengradomkit
C Sunanta
G Zhang
G.O. Aboge
Y. Nishikawa
I. Igarashi
X. Xuan
spellingShingle T. Inpankaew
S. Jittapalapong
J. Phasuk
N. Pinyopanuwut
W. Chimnoi
C. Kengradomkit
C Sunanta
G Zhang
G.O. Aboge
Y. Nishikawa
I. Igarashi
X. Xuan
Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
Onderstepoort Journal of Veterinary Research
author_facet T. Inpankaew
S. Jittapalapong
J. Phasuk
N. Pinyopanuwut
W. Chimnoi
C. Kengradomkit
C Sunanta
G Zhang
G.O. Aboge
Y. Nishikawa
I. Igarashi
X. Xuan
author_sort T. Inpankaew
title Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
title_short Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
title_full Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
title_fullStr Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
title_full_unstemmed Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
title_sort seroprevalence of <i>cryptosporidium parvum</i> infection of dairy cows in three northern provinces of thailand determined by enzyme-linked immunosorbent assay using recombinant antigen cpp23
publisher AOSIS
series Onderstepoort Journal of Veterinary Research
issn 0030-2465
2219-0635
publishDate 2009-09-01
description Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4 %, and the seropositive rates for the three provinces were 3.3 % in Chiang Mai, 5.1 % in Chiang Rai and 3 % in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand.
url https://ojvr.org/index.php/ojvr/article/view/40
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