Effect of Ganshuang granules on lipid metabolism in a new tissue-engineering model of nonalcoholic fatty liver disease

• Main Author: ZHANG Yizhi
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Clinical Hepatology 2020-07-01
• Series: Linchuang Gandanbing Zazhi
• Online Access: http://www.lcgdbzz.org/qk_content.asp?id=10880

ObjectiveTo investigate the effect of Ganshuang granules (GSG) intervention on lipid metabolism in a new tissue-engineering model of nonalcoholic fatty liver disease (NAFLD) and its mechanism. MethodsThe liver of Sprague-Dawley rats was decellularized into collagen scaffolds, and human HepG2 cells were used to recellularize the scaffolds to obtain the tissue-engineering (TE) liver as normal control group. The TE liver was perfused with high-fat medium containing free fatty acid (FFA) to establish a model of NAFLD (FFA group), and this model was further treated with GSG extract to establish a FFA+GSG group. These groups were compared in terms of the content of triglyceride (TG) and the mRNA expression of PDK4 in the liver, and the mRNA expression of enzymes associated with lipid metabolism was compared between the FFA group and the FFA+GSG group; oil red O staining was used to evaluate liver pathology. The t-test was used for comparison of continuous data between groups. ResultsCompared with the normal control group, the FFA group had significantly higher content of TG (t=4.842, P=0.004 7) and mRNA expression of PDK4 (t=2.784, P=0.031 8). Compared with the FFA group, the FFA+GSG group had significant reductions in the content of TG in cells (t=0.055, P=0.003 7), the expression of PDK4 (t=3.761, P=0.009 4), and the mRNA expression of fatty acid translocase, fatty acid-binding protein 1, ATP citrate lyase, acetyl-CoA carboxylase, fatty acid synthase, fatty acid desaturase 2, 1-acylglycerol-3-phosphate O-acyltransferase 5, and apolipoprotein B (t=6.552, 4.944, 2.689, 4.524, 6.040, 3.758, 4.443, and 3.032, P=0.007 2, 0.001 1, 0.027 6, 0.020 2, 0.009 1, 0.005 6, 0.047 1, and 0.016 3). Oil red O staining showed significant reductions in the number of lipid droplets in hepatocytes and the degree of hepatocyte fatty degeneration. ConclusionGSG can reduce the intake of FFA, the de novo synthesis of fatty acid, and the generation and deposition of TG in hepatocytes in the model of NAFLD and thus improve dyslipidemia.