Cloning and Expression of Endoglucanase Gene from Thermophilic Bacteria Bacillus sp. RP1

Cloning and Expression of Endoglucanase Gene from Thermophilic Bacteria Bacillus sp. RP1

An endoglucanase gene from glycoside hydrolase family 5, had been isolated from Bacillus sp. RP1 and cloned into Escherichia coli. The cloned gene comprised the promoter, coding sequence and terminator of the gene.  This gene encoded a protein with 499 amino acid residues (Mr=55.2 kDa) with a typic...

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Bibliographic Details
Main Authors: MAELITA RAMDANI MOEIS, DESSY NATALIA, RAHMA WIDYA NINGRUM, ARI DWIJAYANTI
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2015-03-01
Series:Microbiology Indonesia
Subjects:
Bacillus sp. RP1
recombinant endoglucanase
native promoter
native signal peptide
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/307

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