Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests

<p>Abstract</p> <p>Background</p> <p>The brown planthopper (BPH), <it>Nilaparvata lugens </it>(Hemiptera, Delphacidae), is a serious insect pests of rice plants. Major means of BPH control are application of agricultural chemicals and cultivation of BPH resi...

Full description

Bibliographic Details
Main Authors: Zhang Qiang, Matsui Kageaki, Koizumi Yoko, Kawai Sawako, Noda Hiroaki, Furukawa Shigetoyo, Shimomura Michihiko, Mita Kazuei
Format: Article
Language:English
Published: BMC 2008-03-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/9/117
id doaj-fe7b81c698094dfcbb2fe22db506cb0f
record_format Article
spelling doaj-fe7b81c698094dfcbb2fe22db506cb0f2020-11-25T01:01:00ZengBMCBMC Genomics1471-21642008-03-019111710.1186/1471-2164-9-117Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pestsZhang QiangMatsui KageakiKoizumi YokoKawai SawakoNoda HiroakiFurukawa ShigetoyoShimomura MichihikoMita Kazuei<p>Abstract</p> <p>Background</p> <p>The brown planthopper (BPH), <it>Nilaparvata lugens </it>(Hemiptera, Delphacidae), is a serious insect pests of rice plants. Major means of BPH control are application of agricultural chemicals and cultivation of BPH resistant rice varieties. Nevertheless, BPH strains that are resistant to agricultural chemicals have developed, and BPH strains have appeared that are virulent against the resistant rice varieties. Expressed sequence tag (EST) analysis and related applications are useful to elucidate the mechanisms of resistance and virulence and to reveal physiological aspects of this non-model insect, with its poorly understood genetic background.</p> <p>Results</p> <p>More than 37,000 high-quality ESTs, excluding sequences of mitochondrial genome, microbial genomes, and rDNA, have been produced from 18 libraries of various BPH tissues and stages. About 10,200 clusters have been made from whole EST sequences, with average EST size of 627 bp. Among the top ten most abundantly expressed genes, three are unique and show no homology in BLAST searches. The actin gene was highly expressed in BPH, especially in the thorax. Tissue-specifically expressed genes were extracted based on the expression frequency among the libraries. An EST database is available at our web site.</p> <p>Conclusion</p> <p>The EST library will provide useful information for transcriptional analyses, proteomic analyses, and gene functional analyses of BPH. Moreover, specific genes for hemimetabolous insects will be identified. The microarray fabricated based on the EST information will be useful for finding genes related to agricultural and biological problems related to this pest.</p> http://www.biomedcentral.com/1471-2164/9/117
collection DOAJ
language English
format Article
sources DOAJ
author Zhang Qiang
Matsui Kageaki
Koizumi Yoko
Kawai Sawako
Noda Hiroaki
Furukawa Shigetoyo
Shimomura Michihiko
Mita Kazuei
spellingShingle Zhang Qiang
Matsui Kageaki
Koizumi Yoko
Kawai Sawako
Noda Hiroaki
Furukawa Shigetoyo
Shimomura Michihiko
Mita Kazuei
Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
BMC Genomics
author_facet Zhang Qiang
Matsui Kageaki
Koizumi Yoko
Kawai Sawako
Noda Hiroaki
Furukawa Shigetoyo
Shimomura Michihiko
Mita Kazuei
author_sort Zhang Qiang
title Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
title_short Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
title_full Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
title_fullStr Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
title_full_unstemmed Annotated ESTs from various tissues of the brown planthopper <it>Nilaparvata lugens</it>: A genomic resource for studying agricultural pests
title_sort annotated ests from various tissues of the brown planthopper <it>nilaparvata lugens</it>: a genomic resource for studying agricultural pests
publisher BMC
series BMC Genomics
issn 1471-2164
publishDate 2008-03-01
description <p>Abstract</p> <p>Background</p> <p>The brown planthopper (BPH), <it>Nilaparvata lugens </it>(Hemiptera, Delphacidae), is a serious insect pests of rice plants. Major means of BPH control are application of agricultural chemicals and cultivation of BPH resistant rice varieties. Nevertheless, BPH strains that are resistant to agricultural chemicals have developed, and BPH strains have appeared that are virulent against the resistant rice varieties. Expressed sequence tag (EST) analysis and related applications are useful to elucidate the mechanisms of resistance and virulence and to reveal physiological aspects of this non-model insect, with its poorly understood genetic background.</p> <p>Results</p> <p>More than 37,000 high-quality ESTs, excluding sequences of mitochondrial genome, microbial genomes, and rDNA, have been produced from 18 libraries of various BPH tissues and stages. About 10,200 clusters have been made from whole EST sequences, with average EST size of 627 bp. Among the top ten most abundantly expressed genes, three are unique and show no homology in BLAST searches. The actin gene was highly expressed in BPH, especially in the thorax. Tissue-specifically expressed genes were extracted based on the expression frequency among the libraries. An EST database is available at our web site.</p> <p>Conclusion</p> <p>The EST library will provide useful information for transcriptional analyses, proteomic analyses, and gene functional analyses of BPH. Moreover, specific genes for hemimetabolous insects will be identified. The microarray fabricated based on the EST information will be useful for finding genes related to agricultural and biological problems related to this pest.</p>
url http://www.biomedcentral.com/1471-2164/9/117
work_keys_str_mv AT zhangqiang annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT matsuikageaki annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT koizumiyoko annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT kawaisawako annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT nodahiroaki annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT furukawashigetoyo annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT shimomuramichihiko annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
AT mitakazuei annotatedestsfromvarioustissuesofthebrownplanthopperitnilaparvatalugensitagenomicresourceforstudyingagriculturalpests
_version_ 1725211366725255168