Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2

Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2

Tetrahydrobiopterin (BH4) was released from PC 12 cells to the extracellular fluid. We found that the BH4 outward flow from the cells placed in Hanks medium was increased when NaCl concentration of the medium was decreased. Increase in the BH4 out flow was not observed when NaCl in the Hanks medium...

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Main Authors: Nakanishi Nobuo, Kurihara Kinji, Tomomura Akito, Tonosaki Keiichi, Hattori Yoshiyuki, Oguro Kazuya, Hasegawa Hiroyuki
Format: Article
Language:English
Published: De Gruyter 2005-02-01
Series:Pteridines
Subjects:
tetrahydrobiopterin
water transport
aquaporin inhibitor
pc 12 cells
Online Access:https://doi.org/10.1515/pteridines.2005.16.1.1
id doaj-fe5bcc8f3463473f8eeba117e0c4ff8d
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spelling doaj-fe5bcc8f3463473f8eeba117e0c4ff8d2021-09-05T14:00:02ZengDe GruyterPteridines0933-48072195-47202005-02-011611510.1515/pteridines.2005.16.1.1Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2Nakanishi Nobuo0Kurihara Kinji1Tomomura Akito2Tonosaki Keiichi3Hattori Yoshiyuki4Oguro Kazuya5Hasegawa Hiroyuki6Department of Biochemistry, Meikai University School of Dentistry, Sakado, Saitama 350-0283, JapanDepartment of Physiology, Meikai University School of Dentistry, Sakado, Saitama 350-0283, JapanDepartment of Biochemistry, Meikai University School of Dentistry, Sakado, Saitama 350-0283, JapanDepartment of Physiology, Meikai University School of Dentistry, Sakado, Saitama 350-0283, JapanDepartment of Endocrinology and Metabolism, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293, Japandepartment of Biosciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, Japandepartment of Biosciences, Teikyo University of Science and Technology, Uenohara, Yamanashi 409-0193, JapanTetrahydrobiopterin (BH4) was released from PC 12 cells to the extracellular fluid. We found that the BH4 outward flow from the cells placed in Hanks medium was increased when NaCl concentration of the medium was decreased. Increase in the BH4 out flow was not observed when NaCl in the Hanks medium was substituted with sodium glutamate (0.14 M), choline chloride (0.14 M) or sucrosc (0.25 M). HgCl2, an inhibitor of water channel, aquaporin, prevented the increase in BH4 out flow caused by the hypotonic medium. The results suggested the presence of a site in BH4 transport which was stimulated by osmotic pressure and/or water influx.https://doi.org/10.1515/pteridines.2005.16.1.1tetrahydrobiopterinwater transportaquaporin inhibitorpc 12 cells
collection DOAJ
language English
format Article
sources DOAJ
author Nakanishi Nobuo
Kurihara Kinji
Tomomura Akito
Tonosaki Keiichi
Hattori Yoshiyuki
Oguro Kazuya
Hasegawa Hiroyuki
spellingShingle Nakanishi Nobuo
Kurihara Kinji
Tomomura Akito
Tonosaki Keiichi
Hattori Yoshiyuki
Oguro Kazuya
Hasegawa Hiroyuki
Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
Pteridines
tetrahydrobiopterin
water transport
aquaporin inhibitor
pc 12 cells
author_facet Nakanishi Nobuo
Kurihara Kinji
Tomomura Akito
Tonosaki Keiichi
Hattori Yoshiyuki
Oguro Kazuya
Hasegawa Hiroyuki
author_sort Nakanishi Nobuo
title Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
title_short Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
title_full Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
title_fullStr Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
title_full_unstemmed Increase in Tetrahydrobiopterin Release from PC 12 Cells under Hypotonic Culture Conditions is Inhibited by HgCl2
title_sort increase in tetrahydrobiopterin release from pc 12 cells under hypotonic culture conditions is inhibited by hgcl2
publisher De Gruyter
series Pteridines
issn 0933-4807
2195-4720
publishDate 2005-02-01
description Tetrahydrobiopterin (BH4) was released from PC 12 cells to the extracellular fluid. We found that the BH4 outward flow from the cells placed in Hanks medium was increased when NaCl concentration of the medium was decreased. Increase in the BH4 out flow was not observed when NaCl in the Hanks medium was substituted with sodium glutamate (0.14 M), choline chloride (0.14 M) or sucrosc (0.25 M). HgCl2, an inhibitor of water channel, aquaporin, prevented the increase in BH4 out flow caused by the hypotonic medium. The results suggested the presence of a site in BH4 transport which was stimulated by osmotic pressure and/or water influx.
topic tetrahydrobiopterin
water transport
aquaporin inhibitor
pc 12 cells
url https://doi.org/10.1515/pteridines.2005.16.1.1
work_keys_str_mv AT nakanishinobuo increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT kuriharakinji increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT tomomuraakito increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT tonosakikeiichi increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT hattoriyoshiyuki increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT ogurokazuya increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
AT hasegawahiroyuki increaseintetrahydrobiopterinreleasefrompc12cellsunderhypotoniccultureconditionsisinhibitedbyhgcl2
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