Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells

Abstract.: Prostaglandin E2 (PGE2) was shown to induce neuronal death in the CNS. To characterize the neurotoxicity of PGE2 and E-prostanoid receptors (EP) in motor neurons, we investigated PGE2-induced cell death and the type(s) of EP responsible for mediating it in NSC-34, a motor neuron-like cell...

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Main Authors:	Hiroko Miyagishi, Yasuhiro Kosuge, Yuki Yoneoka, Maiko Ozone, Manami Endo, Nobuhiro Osada, Kumiko Ishige, Kuniko Kusama-Eguchi, Yoshihisa Ito
Format:	Article
Language:	English
Published:	Elsevier 2013-01-01
Series:	Journal of Pharmacological Sciences
Online Access:	http://www.sciencedirect.com/science/article/pii/S1347861319303652

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spelling	doaj-fdbaf0e72e82462baa8525f7528067472020-11-25T02:06:07ZengElsevierJournal of Pharmacological Sciences1347-86132013-01-011214347350Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 CellsHiroko Miyagishi0Yasuhiro Kosuge1Yuki Yoneoka2Maiko Ozone3Manami Endo4Nobuhiro Osada5Kumiko Ishige6Kuniko Kusama-Eguchi7Yoshihisa Ito8Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Biochemistry, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan; Corresponding author. ito.yoshihisa@nihon-u.ac.jpAbstract.: Prostaglandin E2 (PGE2) was shown to induce neuronal death in the CNS. To characterize the neurotoxicity of PGE2 and E-prostanoid receptors (EP) in motor neurons, we investigated PGE2-induced cell death and the type(s) of EP responsible for mediating it in NSC-34, a motor neuron-like cell line. Immunoblotting studies showed that EP2 and EP3 were dominantly expressed in NSC-34 cells and motor neurons in mice. Exposure to PGE2 and butaprost, an EP2 agonist, but not sulprostone, an EP1/3 agonist, resulted in decreased viability of these cells. These results suggest that PGE2 induces cell death by activation of EP2 in NSC-34 cells. Keywords:: prostaglandin E2, E-prostanoid receptor, motor neuronhttp://www.sciencedirect.com/science/article/pii/S1347861319303652
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hiroko Miyagishi Yasuhiro Kosuge Yuki Yoneoka Maiko Ozone Manami Endo Nobuhiro Osada Kumiko Ishige Kuniko Kusama-Eguchi Yoshihisa Ito
spellingShingle	Hiroko Miyagishi Yasuhiro Kosuge Yuki Yoneoka Maiko Ozone Manami Endo Nobuhiro Osada Kumiko Ishige Kuniko Kusama-Eguchi Yoshihisa Ito Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells Journal of Pharmacological Sciences
author_facet	Hiroko Miyagishi Yasuhiro Kosuge Yuki Yoneoka Maiko Ozone Manami Endo Nobuhiro Osada Kumiko Ishige Kuniko Kusama-Eguchi Yoshihisa Ito
author_sort	Hiroko Miyagishi
title	Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells
title_short	Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells
title_full	Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells
title_fullStr	Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells
title_full_unstemmed	Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells
title_sort	prostaglandin e2-induced cell death is mediated by activation of ep2 receptors in motor neuron-like nsc-34 cells
publisher	Elsevier
series	Journal of Pharmacological Sciences
issn	1347-8613
publishDate	2013-01-01
description	Abstract.: Prostaglandin E2 (PGE2) was shown to induce neuronal death in the CNS. To characterize the neurotoxicity of PGE2 and E-prostanoid receptors (EP) in motor neurons, we investigated PGE2-induced cell death and the type(s) of EP responsible for mediating it in NSC-34, a motor neuron-like cell line. Immunoblotting studies showed that EP2 and EP3 were dominantly expressed in NSC-34 cells and motor neurons in mice. Exposure to PGE2 and butaprost, an EP2 agonist, but not sulprostone, an EP1/3 agonist, resulted in decreased viability of these cells. These results suggest that PGE2 induces cell death by activation of EP2 in NSC-34 cells. Keywords:: prostaglandin E2, E-prostanoid receptor, motor neuron
url	http://www.sciencedirect.com/science/article/pii/S1347861319303652
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Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells

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