Prostaglandin E2-Induced Cell Death is Mediated by Activation of EP2 Receptors in Motor Neuron-like NSC-34 Cells

• Main Authors: Hiroko Miyagishi, Yasuhiro Kosuge, Yuki Yoneoka, Maiko Ozone, Manami Endo, Nobuhiro Osada, Kumiko Ishige, Kuniko Kusama-Eguchi, Yoshihisa Ito
• Format: Article
• Language: English
• Published: Elsevier 2013-01-01
• Series: Journal of Pharmacological Sciences
• Online Access: http://www.sciencedirect.com/science/article/pii/S1347861319303652
• ISSN: 1347-8613

Abstract.: Prostaglandin E2 (PGE2) was shown to induce neuronal death in the CNS. To characterize the neurotoxicity of PGE2 and E-prostanoid receptors (EP) in motor neurons, we investigated PGE2-induced cell death and the type(s) of EP responsible for mediating it in NSC-34, a motor neuron-like cell line. Immunoblotting studies showed that EP2 and EP3 were dominantly expressed in NSC-34 cells and motor neurons in mice. Exposure to PGE2 and butaprost, an EP2 agonist, but not sulprostone, an EP1/3 agonist, resulted in decreased viability of these cells. These results suggest that PGE2 induces cell death by activation of EP2 in NSC-34 cells. Keywords:: prostaglandin E2, E-prostanoid receptor, motor neuron