Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies.
INTRODUCTION: A prerequisite to accurate interpretation of RQ-PCR data is robust data normalization. A commonly used method is to compare the cycle threshold (CT) of target miRNAs with those of a stably expressed endogenous (EC) miRNA(s) from the same sample. Despite the large number of studies repo...
Main Authors: | Ailbhe M McDermott, Michael J Kerin, Nicola Miller |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3877087?pdf=render |
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