Morpho-Physio-Biochemical and Molecular Responses of Maize Hybrids to Salinity and Waterlogging during Stress and Recovery Phase

• Main Authors: Umer Mahmood, Saddam Hussain, Sadam Hussain, Basharat Ali, Umair Ashraf, Shahid Zamir, Sami Asir Al-Robai, Fatima Omari Alzahrani, Christophe Hano, Mohamed A. El-Esawi
• Format: Article
• Language: English
• Published: MDPI AG 2021-07-01
• Series: Plants
• Subjects: plant growth; waterlogging; antioxidant machinery; ROS production; chlorophyll pigments; salt stress
• Online Access: https://www.mdpi.com/2223-7747/10/7/1345

Maize is one of the most economically important cereal crops worldwide. Salinity coupled with waterlogging is a major challenge for successful crop production. Understanding the underlying mechanisms and impacts of individual and combined salinity and waterlogging stress on the morpho-physio-biochemical and molecular responses and oxidative metabolism of maize during stress and recovery periods is essential. The present study was carried out to assess the response of four hybrid maize cultivars viz. DK-6142, FH-1231, FH-949, and MALKA-2016 under individual and combined salinity and waterlogging conditions. The treatments comprised the control (no stress), NaCl (salinity with 10 dSm⁻¹), WL (waterlogged conditions with 3 cm flooding), and NaCl + WL (combined salinity and waterlogging stress). The data regarding morpho-physiological attributes were collected at 22 days after sowing (DAS; stress phase) and 30 DAS (recovery phase). The results revealed that both stresses, either individually or in combination, substantially reduced the root-shoot length, root-shoot fresh and dry weights, leaf width, and the number of leaves per plant as well as the leaf chlorophyll (Chl) and carotenoids contents; however, the inhibitory effects were more severe in combined stresses than for individual stress factors in many cultivars. Both individual and combined stress conditions enhanced hydrogen peroxide (H₂O₂) accumulation, whereas the antioxidant enzyme activities, i.e., superoxide dismutase (SOD), peroxidase (POD) catalase (CAT), and ascorbate peroxidase (APX), remained higher under stress conditions compared to the control. The expression levels of antioxidant genes (<i>CAT</i> and <i>POD</i>) were also upregulated under stress conditions. All of the cultivars recovered better from individual stresses than combined stress conditions; however, the hybrid DK-6142 performed better than the other maize hybrids under stress conditions and showed faster recovery.