Effects of 5-aza-2´-deoxycytidine on biological parameters of Achroia grisella F. (Lepidoptera: Pyralidae)
The non-target effects of 5-aza-2´-deoxycytidine (5-aza-dC), an epigenetically effective agent, were assessed on different life-history traits of two successive generations of Achroia grisella F. (Lepidoptera: Pyralidae) by trophic exposure. The results did not reveal any prominent effec...
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Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2018-01-01
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Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2018/0354-46641700034S.pdf |
Summary: | The non-target effects of 5-aza-2´-deoxycytidine (5-aza-dC), an
epigenetically effective agent, were assessed on different life-history
traits of two successive generations of Achroia grisella F. (Lepidoptera:
Pyralidae) by trophic exposure. The results did not reveal any prominent
effect of 5-Aza-dC on emergence times and morphological disorders of
offspring of both sexes, and dry weight of F1 females (except for 0.1 mg/ml)
and males according to controls. However, 5-Aza-dC caused a considerable
decrease in wet weight of F1 females at >0.1 mg/mL and in F1 males only at
0.5 mg/mL. The mean longevity of F1 and F2 females was almost unchanged
after exposure to 5-Aza-dC treatment. However, the longevity was
considerably shorter, by 16% at a dose of 0.75 mg/mL for F1 males and 28%
longer at 1.0 mg/mL for F2 males with respect to the controls. When the two
generations were compared with each other in terms of adult longevity, the
differences were not significant for the longevity of females, whereas F2
males lived significantly longer than F1 males in all groups except for the
control and 0.5 mg/mL groups. 5-Aza-dC also markedly decreased the total
number of both offspring but no dose-related alterations were observed.
Analysis of the data for the number of viable and damaged eggs laid per F1
females revealed that 5-Aza-dC adversely affected the reproductive potential
of A. grisella based on daily and three-day observations. The most striking
effect was a decline in fecundity of females by 57% at 1.0 mg/mL. These
observations suggest that 5-Aza-dC has a negative effect on developing moth
progeny across trophic levels. |
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ISSN: | 0354-4664 1821-4339 |