Study of polymorphism of Myostatin gene and its association with growth traits in lori sheep

• Main Authors: Fatemeh Bahramzadeh, Gholamreza Dashab, Masoud Alipana, Mohammad Rokouei, Sadegh Asadollaei
• Format: Article
• Language: fas
• Published: Ferdowsi University of Mashhad 2015-04-01
• Series: پژوهشهای علوم دامی ایران
• Subjects: Myostatin; Lori sheep; Polymorphism; Heterozygosity
• Online Access: http://ijasr.um.ac.ir/index.php/animal/article/view/26789

Myostatin gene or growth and differentiation factor-8 inhibits the puberty and development of skeletal muscles and it could change the size of some muscles such as double muscle, if the mutation occurs. One of the most important indigenous sheep in Iran, Lori sheep, possess high genetic potential for meat production. Therefore, the study of some genes affecting meat production would be of utmost important in selection programs. A total of 50 Lori sheep have been randomly selected and blood sampling was done to investigate the polymorphism of Myostatin gene and its relation to some economical traits including birth weight, weaning weight and 6-month lambs weight. Genomic DNA was extracted by the Denazyst kit. DNA quantity and quality was estimated by using espectophotometry and agarose electrophoresis. The specific primers were designed to assess the presence of potential polymorphism and then target area was amplified. After confirmation of 373bp fragment from exon 3 of Myostatin gene on the agarose gel, the genotype determination was performed using restriction enzyme HaeІІІ. The results showed that the Mm and mm genotypes were detected in the samples. The frequency of M and m alleles was 8.75 % and 91.25 %, respectively. No significant relationship was observed between genotypes of Myostatin gene and growth traits, except of the birth weight. In conclusion, the Myostatin locus could not considered as a promising marker for growth traits in Lori sheep.