Gene Cloning of Penicillin V Acylase from Bacillus sp BAC4 by Genomic Library
This research was aimed to clone and identify penicillin V acylase (PVA) gene of Bacillus sp. BAC4 by genomic library. Chromosome DNA of Bacillus sp. BAC4 was isolated by Wang method. pHB201 of E. coli was isolated by alkali lyses method. Recombinant DNA of Bacillus sp. BAC4 chromosome fragment and...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MBI & UNS Solo
2004-01-01
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| Series: | Biodiversitas |
| Subjects: | |
| Online Access: | http://biodiversitas.mipa.uns.ac.id/D/D0501/D050101.pdf |