Imobilização de enzimas a partir de "kit" comercial: determinação de parâmetros metabólicos em sangue animal empregando multicomutação em fluxo Enzyme immobilization using a commercial kit: determination of metabolic parameters in animal blood employing a multicommutation flow system

• Main Authors: Cherrine Kelce Pires, Boaventura Freire dos Reis
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2005-06-01
• Series: Química Nova
• Subjects: enzymatic reactions; flow injection analysis; commercial kit
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300010

<abstract language="eng">Automatic flow procedures based on the multicommutation concept, dedicated to the determination of 3-hydroxybutyrate, glucose and cholesterol are proposed. The enzymes were immobilized on glass beads and packed into mini-columns that were coupled to a flow system. Sampling throughputs of 55, 40 and 40 determinations per hour, linear response from 10 to 150, 50 to 600, 25 to 125 mg L-1, detection limits of 1.5, 14 and 4 mg L-1 and relative standard deviations of 1, 2 and 2% for 3-hydroxybutyrate, glucose and cholesterol, respectively, were achieved.