Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa

This study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and We...

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Main Authors:	Eiman Alshawa, Mohammed Laqqan, Mathias Montenarh, Mohamad Eid Hammadeh
Format:	Article
Language:	English
Published:	Elsevier 2019-01-01
Series:	Toxicology Reports
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214750019303221

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spelling	doaj-fccdd474d6e143c982322a5e1728ebe32020-11-25T01:30:11ZengElsevierToxicology Reports2214-75002019-01-016819824Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoaEiman Alshawa0Mohammed Laqqan1Mathias Montenarh2Mohamad Eid Hammadeh3Department of Obstetrics, Gynecology & Assisted Reproduction Laboratory, Saarland University, Homburg, GermanyDepartment of Obstetrics, Gynecology & Assisted Reproduction Laboratory, Saarland University, Homburg, Germany; Corresponding author.Medical Biochemistry and Molecular Biology, Saarland University, Homburg, GermanyDepartment of Obstetrics, Gynecology & Assisted Reproduction Laboratory, Saarland University, Homburg, GermanyThis study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and Western blot analysis. The results showed a significant reduction in the expression levels of CATSPER2 and TEKT2 in the cryopreserved compared to the fresh samples (P = 0.0039 and P = 0.0166, respectively), and the results showed down-regulation in the expression level of CATSPER2 and TEKT2 genes between the study groups. Moreover, the protein levels of the CatSper2 and Tektin2 were lower in cryopreserved samples compared to fresh samples (P = 0.0001). In conclusion, the reduction in the proteins level and expression level of the CATSPER2 and TEKT2 in cryopreserved samples could be used as an indicator of sperm motility loss. Keywords: CATSPER2, Cryopreservation, Spermatozoa, TEKT2http://www.sciencedirect.com/science/article/pii/S2214750019303221
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Eiman Alshawa Mohammed Laqqan Mathias Montenarh Mohamad Eid Hammadeh
spellingShingle	Eiman Alshawa Mohammed Laqqan Mathias Montenarh Mohamad Eid Hammadeh Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa Toxicology Reports
author_facet	Eiman Alshawa Mohammed Laqqan Mathias Montenarh Mohamad Eid Hammadeh
author_sort	Eiman Alshawa
title	Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_short	Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_full	Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_fullStr	Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_full_unstemmed	Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
title_sort	influence of cryopreservation on the catsper2 and tekt2 expression levels and protein levels in human spermatozoa
publisher	Elsevier
series	Toxicology Reports
issn	2214-7500
publishDate	2019-01-01
description	This study designed to assess the expression level of CATSPER2 and TEKT2 and to evaluate the levels of CatSper2 and Tektin2 proteins in human spermatozoa before and after cryopreservation. One hundred and twenty semen samples were included in this study. All the samples were subjected to qPCR and Western blot analysis. The results showed a significant reduction in the expression levels of CATSPER2 and TEKT2 in the cryopreserved compared to the fresh samples (P = 0.0039 and P = 0.0166, respectively), and the results showed down-regulation in the expression level of CATSPER2 and TEKT2 genes between the study groups. Moreover, the protein levels of the CatSper2 and Tektin2 were lower in cryopreserved samples compared to fresh samples (P = 0.0001). In conclusion, the reduction in the proteins level and expression level of the CATSPER2 and TEKT2 in cryopreserved samples could be used as an indicator of sperm motility loss. Keywords: CATSPER2, Cryopreservation, Spermatozoa, TEKT2
url	http://www.sciencedirect.com/science/article/pii/S2214750019303221
work_keys_str_mv	AT eimanalshawa influenceofcryopreservationonthecatsper2andtekt2expressionlevelsandproteinlevelsinhumanspermatozoa AT mohammedlaqqan influenceofcryopreservationonthecatsper2andtekt2expressionlevelsandproteinlevelsinhumanspermatozoa AT mathiasmontenarh influenceofcryopreservationonthecatsper2andtekt2expressionlevelsandproteinlevelsinhumanspermatozoa AT mohamadeidhammadeh influenceofcryopreservationonthecatsper2andtekt2expressionlevelsandproteinlevelsinhumanspermatozoa
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Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa

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