Letter to the editor: blood processing and sample storage have negligible effects on methylation

Abstract DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that...

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Main Authors:	Kira Groen, Rodney A. Lea, Vicki E. Maltby, Rodney J. Scott, Jeannette Lechner-Scott
Format:	Article
Language:	English
Published:	BMC 2018-02-01
Series:	Clinical Epigenetics
Subjects:	Methylation Blood Processing Storage DNA extraction
Online Access:	http://link.springer.com/article/10.1186/s13148-018-0455-6

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spelling	doaj-fcc95fb5cb614bbc8d14c341b0e4c6932020-11-25T00:44:48ZengBMCClinical Epigenetics1868-70751868-70832018-02-011011510.1186/s13148-018-0455-6Letter to the editor: blood processing and sample storage have negligible effects on methylationKira Groen0Rodney A. Lea1Vicki E. Maltby2Rodney J. Scott3Jeannette Lechner-Scott4School of Medicine and Public Health, University of NewcastleCentre for Information Based Medicine, Hunter Medical Research InstituteSchool of Medicine and Public Health, University of NewcastleCentre for Information Based Medicine, Hunter Medical Research InstituteSchool of Medicine and Public Health, University of NewcastleAbstract DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variation in collection, storage, and processing of blood DNA confers negligible effects on both global methylation and methylation status of specific genes. These results are concordant with studies that have investigated the effect of sample storage and processing on methylation in other tissues, such as tumour, sperm, and placenta samples.http://link.springer.com/article/10.1186/s13148-018-0455-6MethylationBloodProcessingStorageDNA extraction
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kira Groen Rodney A. Lea Vicki E. Maltby Rodney J. Scott Jeannette Lechner-Scott
spellingShingle	Kira Groen Rodney A. Lea Vicki E. Maltby Rodney J. Scott Jeannette Lechner-Scott Letter to the editor: blood processing and sample storage have negligible effects on methylation Clinical Epigenetics Methylation Blood Processing Storage DNA extraction
author_facet	Kira Groen Rodney A. Lea Vicki E. Maltby Rodney J. Scott Jeannette Lechner-Scott
author_sort	Kira Groen
title	Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_short	Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_full	Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_fullStr	Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_full_unstemmed	Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_sort	letter to the editor: blood processing and sample storage have negligible effects on methylation
publisher	BMC
series	Clinical Epigenetics
issn	1868-7075 1868-7083
publishDate	2018-02-01
description	Abstract DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variation in collection, storage, and processing of blood DNA confers negligible effects on both global methylation and methylation status of specific genes. These results are concordant with studies that have investigated the effect of sample storage and processing on methylation in other tissues, such as tumour, sperm, and placenta samples.
topic	Methylation Blood Processing Storage DNA extraction
url	http://link.springer.com/article/10.1186/s13148-018-0455-6
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Letter to the editor: blood processing and sample storage have negligible effects on methylation

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