Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging

Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.

Bibliographic Details
Main Authors:	Henry Pinkard, Hratch Baghdassarian, Adriana Mujal, Ed Roberts, Kenneth H. Hu, Daniel Haim Friedman, Ivana Malenica, Taylor Shagam, Adam Fries, Kaitlin Corbin, Matthew F. Krummel, Laura Waller
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-22246-5

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spelling	doaj-fcc73bb7eff54047abea2f8a85694bb02021-03-28T11:12:03ZengNature Publishing GroupNature Communications2041-17232021-03-0112111410.1038/s41467-021-22246-5Learned adaptive multiphoton illumination microscopy for large-scale immune response imagingHenry Pinkard0Hratch Baghdassarian1Adriana Mujal2Ed Roberts3Kenneth H. Hu4Daniel Haim Friedman5Ivana Malenica6Taylor Shagam7Adam Fries8Kaitlin Corbin9Matthew F. Krummel10Laura Waller11Department of Electrical Engineering and Computer Sciences, University of CaliforniaDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoDepartment of Bioengineering, University of CaliforniaBerkeley Institute for Data ScienceDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoDepartment of Pathology, University of California, San FranciscoComputational Biology Graduate Group, University of CaliforniaMultiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.https://doi.org/10.1038/s41467-021-22246-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Henry Pinkard Hratch Baghdassarian Adriana Mujal Ed Roberts Kenneth H. Hu Daniel Haim Friedman Ivana Malenica Taylor Shagam Adam Fries Kaitlin Corbin Matthew F. Krummel Laura Waller
spellingShingle	Henry Pinkard Hratch Baghdassarian Adriana Mujal Ed Roberts Kenneth H. Hu Daniel Haim Friedman Ivana Malenica Taylor Shagam Adam Fries Kaitlin Corbin Matthew F. Krummel Laura Waller Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging Nature Communications
author_facet	Henry Pinkard Hratch Baghdassarian Adriana Mujal Ed Roberts Kenneth H. Hu Daniel Haim Friedman Ivana Malenica Taylor Shagam Adam Fries Kaitlin Corbin Matthew F. Krummel Laura Waller
author_sort	Henry Pinkard
title	Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
title_short	Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
title_full	Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
title_fullStr	Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
title_full_unstemmed	Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
title_sort	learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-03-01
description	Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.
url	https://doi.org/10.1038/s41467-021-22246-5
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Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging

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