Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging
Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.
Bibliographic Details
| Main Authors: |
Henry Pinkard,
Hratch Baghdassarian,
Adriana Mujal,
Ed Roberts,
Kenneth H. Hu,
Daniel Haim Friedman,
Ivana Malenica,
Taylor Shagam,
Adam Fries,
Kaitlin Corbin,
Matthew F. Krummel,
Laura Waller |
|---|
| Format: | Article
|
|---|
| Language: | English |
|---|
| Published: |
Nature Publishing Group
2021-03-01
|
|---|
| Series: | Nature Communications
|
|---|
| Online Access: | https://doi.org/10.1038/s41467-021-22246-5
|
|---|
