Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice

• Main Authors: Ligang Zhou, Shu Wang, Zhiyi Chen, Wenxian Sun, Shiqiong Lu, Mingan Wang, Shan Gao, Hao Liu, Weibo Sun, Tijiang Shan
• Format: Article
• Language: English
• Published: MDPI AG 2012-09-01
• Series: International Journal of Molecular Sciences
• Subjects: Villosiclava virens; ustiloxins A and B; false smut balls of rice; determination and analysis; LC-ESI-MS; HPLC
• Online Access: http://www.mdpi.com/1422-0067/13/9/11275

Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.