Laboratory Evolution of GH11 Endoxylanase Through DNA Shuffling: Effects of Distal Residue Substitution on Catalytic Activity and Active Site Architecture

Laboratory Evolution of GH11 Endoxylanase Through DNA Shuffling: Effects of Distal Residue Substitution on Catalytic Activity and Active Site Architecture

Endoxylanase with high specific activity, thermostability, and broad pH adaptability is in huge demand. The mutant library of GH11 endoxylanase was constructed via DNA shuffling by using the catalytic domain of Bacillus amyloliquefaciens xylanase A (BaxA) and Thermomonospora fusca TF xylanase A (Tfx...

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Bibliographic Details
Main Authors: Ming-Qi Liu, Jia-Yi Li, Ashfaq Ur Rehman, Xin Xu, Zhu-Jun Gu, Ruo-Chen Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
xylanase
DNA shuffling
catalytic activity
molecular dynamics simulations
non-covalent interactions (NCI)
xylooligosaccharides
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00350/full

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