A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae [version 2; referees: 3 approved]

Integrating a desired DNA sequence into the yeast genomes is a widely-used genetic manipulation in the budding yeast Saccharomyces cerevisiae. The conventional integration method is to use an integrative plasmid such as pRS or YIplac series as the target DNA carrier. The nature of this method risks...

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Bibliographic Details
Main Authors: Peter W Daniels, Anuradha Mukherjee, Alastair SH Goldman, Bin Hu
Format: Article
Language:English
Published: Wellcome 2018-07-01
Series:Wellcome Open Research
Online Access:https://wellcomeopenresearch.org/articles/3-72/v2