Study to Eliminate the Effect of Hyperbilirubinemia in Measurement of Blood Coagulation Assay

• Main Authors: RAGHUNATHAN R., NEELAMEGAM P., JAMALUDEEN A., MURUGANANTHAN K.
• Format: Article
• Language: English
• Published: IFSA Publishing, S.L. 2013-02-01
• Series: Sensors & Transducers
• Subjects: PT; APTT; Hyperbilirubinemia; ICTERIC; Cirrhosis
• Online Access: http://www.sensorsportal.com/HTML/DIGEST/february_2013/P_1146.pdf
• ISSN: 2306-8515; 1726-5479

In this work a low cost coagulation analyzer is developed using LPC 2138 Microcontroller to determine the values of PT (Prothrombin Time) and APTT (Activated partial Thomboplastin Time) in samples with Hyperbilirubinimea (High Bilirubin) ranging from 0-22.0 mg/dl. Most of the conventional Instruments are able to detect the clotting time only when the bilirubin level in the sample is within the range of 0-5.0 mg/dl. It is really a challenge to overcome the interference due to high bilirubin (yellow color) and determine the values of PT and APTT in ICTERIC (jaundice) or liver cirrhosis patients. In the developed instrument yellow light (LED 590 nm) having a minimum absorbance (Beer-Lambert’s Law) while passing through the yellowish sample is used to detect the changes in light intensity while clotting of the sample occurs and thus eliminates the interference of higher bilirubin content (above 4 mg/dl) in blood samples. In the conventional instruments the light (Red -above 630 nm) is absorbed to the maximum and therefore it becomes difficult to detect minute changes in the outcoming light while clotting occurs, limiting its performance. It is observed that the designed Instrument shows the performance of measurement of PT and APTT in 95.5 % samples with hyperbilirubinemia against 31.8 % of samples in COAT-1 analyzer.