Methods for in vitro evaluating antimicrobial activity: A review

In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffu...

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Main Authors: Mounyr Balouiri, Moulay Sadiki, Saad Koraichi Ibnsouda
Format: Article
Language:English
Published: Elsevier 2016-04-01
Series:Journal of Pharmaceutical Analysis
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177915300150
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spelling doaj-fc4b095480df4809b3f3cf2ed93a7bb12021-04-02T09:43:35ZengElsevierJournal of Pharmaceutical Analysis2095-17792016-04-0162717910.1016/j.jpha.2015.11.005Methods for in vitro evaluating antimicrobial activity: A reviewMounyr BalouiriMoulay SadikiSaad Koraichi IbnsoudaIn recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.http://www.sciencedirect.com/science/article/pii/S2095177915300150Thin-layer chromatography (TLC)–bioautographyTime-kill testAntimicrobial gradient methodAgar diffusion method
collection DOAJ
language English
format Article
sources DOAJ
author Mounyr Balouiri
Moulay Sadiki
Saad Koraichi Ibnsouda
spellingShingle Mounyr Balouiri
Moulay Sadiki
Saad Koraichi Ibnsouda
Methods for in vitro evaluating antimicrobial activity: A review
Journal of Pharmaceutical Analysis
Thin-layer chromatography (TLC)–bioautography
Time-kill test
Antimicrobial gradient method
Agar diffusion method
author_facet Mounyr Balouiri
Moulay Sadiki
Saad Koraichi Ibnsouda
author_sort Mounyr Balouiri
title Methods for in vitro evaluating antimicrobial activity: A review
title_short Methods for in vitro evaluating antimicrobial activity: A review
title_full Methods for in vitro evaluating antimicrobial activity: A review
title_fullStr Methods for in vitro evaluating antimicrobial activity: A review
title_full_unstemmed Methods for in vitro evaluating antimicrobial activity: A review
title_sort methods for in vitro evaluating antimicrobial activity: a review
publisher Elsevier
series Journal of Pharmaceutical Analysis
issn 2095-1779
publishDate 2016-04-01
description In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.
topic Thin-layer chromatography (TLC)–bioautography
Time-kill test
Antimicrobial gradient method
Agar diffusion method
url http://www.sciencedirect.com/science/article/pii/S2095177915300150
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