Methods for in vitro evaluating antimicrobial activity: A review
In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffu...
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2016-04-01
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doaj-fc4b095480df4809b3f3cf2ed93a7bb12021-04-02T09:43:35ZengElsevierJournal of Pharmaceutical Analysis2095-17792016-04-0162717910.1016/j.jpha.2015.11.005Methods for in vitro evaluating antimicrobial activity: A reviewMounyr BalouiriMoulay SadikiSaad Koraichi IbnsoudaIn recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.http://www.sciencedirect.com/science/article/pii/S2095177915300150Thin-layer chromatography (TLC)–bioautographyTime-kill testAntimicrobial gradient methodAgar diffusion method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mounyr Balouiri Moulay Sadiki Saad Koraichi Ibnsouda |
spellingShingle |
Mounyr Balouiri Moulay Sadiki Saad Koraichi Ibnsouda Methods for in vitro evaluating antimicrobial activity: A review Journal of Pharmaceutical Analysis Thin-layer chromatography (TLC)–bioautography Time-kill test Antimicrobial gradient method Agar diffusion method |
author_facet |
Mounyr Balouiri Moulay Sadiki Saad Koraichi Ibnsouda |
author_sort |
Mounyr Balouiri |
title |
Methods for in vitro evaluating antimicrobial activity: A review |
title_short |
Methods for in vitro evaluating antimicrobial activity: A review |
title_full |
Methods for in vitro evaluating antimicrobial activity: A review |
title_fullStr |
Methods for in vitro evaluating antimicrobial activity: A review |
title_full_unstemmed |
Methods for in vitro evaluating antimicrobial activity: A review |
title_sort |
methods for in vitro evaluating antimicrobial activity: a review |
publisher |
Elsevier |
series |
Journal of Pharmaceutical Analysis |
issn |
2095-1779 |
publishDate |
2016-04-01 |
description |
In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported. |
topic |
Thin-layer chromatography (TLC)–bioautography Time-kill test Antimicrobial gradient method Agar diffusion method |
url |
http://www.sciencedirect.com/science/article/pii/S2095177915300150 |
work_keys_str_mv |
AT mounyrbalouiri methodsforinvitroevaluatingantimicrobialactivityareview AT moulaysadiki methodsforinvitroevaluatingantimicrobialactivityareview AT saadkoraichiibnsouda methodsforinvitroevaluatingantimicrobialactivityareview |
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