Molecular Basis of Bacterial Host Interactions by Gram-Positive Targeting Bacteriophages

• Main Authors: Matthew Dunne, Mario Hupfeld, Jochen Klumpp, Martin J. Loessner
• Format: Article
• Language: English
• Published: MDPI AG 2018-07-01
• Series: Viruses
• Subjects: gram-positive bacteria; bacteriophage; infection; receptor-binding proteins; phage technology; Listeria monocytogenes; Lactococcus lactis; Bacillus subtilis; Staphylococcus aureus
• Online Access: http://www.mdpi.com/1999-4915/10/8/397

The inherent ability of bacteriophages (phages) to infect specific bacterial hosts makes them ideal candidates to develop into antimicrobial agents for pathogen-specific remediation in food processing, biotechnology, and medicine (e.g., phage therapy). Conversely, phage contaminations of fermentation processes are a major concern to dairy and bioprocessing industries. The first stage of any successful phage infection is adsorption to a bacterial host cell, mediated by receptor-binding proteins (RBPs). As the first point of contact, the binding specificity of phage RBPs is the primary determinant of bacterial host range, and thus defines the remediative potential of a phage for a given bacterium. Co-evolution of RBPs and their bacterial receptors has forced endless adaptation cycles of phage-host interactions, which in turn has created a diverse array of phage adsorption mechanisms utilizing an assortment of RBPs. Over the last decade, these intricate mechanisms have been studied intensely using electron microscopy and X-ray crystallography, providing atomic-level details of this fundamental stage in the phage infection cycle. This review summarizes current knowledge surrounding the molecular basis of host interaction for various socioeconomically important Gram-positive targeting phage RBPs to their protein- and saccharide-based receptors. Special attention is paid to the abundant and best-characterized Siphoviridae family of tailed phages. Unravelling these complex phage-host dynamics is essential to harness the full potential of phage-based technologies, or for generating novel strategies to combat industrial phage contaminations.