Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.

The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxid...

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Main Authors: Yong Hwan Kwon, Nayoung Kim, Ryoung Hee Nam, Ji Hyun Park, Sun Min Lee, Sung Kook Kim, Hye Seung Lee, Yong Sung Kim, Dong Ho Lee
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5207530?pdf=render
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spelling doaj-fc3d59dbf2d44e8eafbd942cf30163762020-11-25T00:08:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016911310.1371/journal.pone.0169113Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.Yong Hwan KwonNayoung KimRyoung Hee NamJi Hyun ParkSun Min LeeSung Kook KimHye Seung LeeYong Sung KimDong Ho LeeThe gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry. The density of the nNOS-positive immunoreactive area also decreased with age in the SMB, circular muscle (CM), and MP. Similarly, the percent of nNOS-positive neuronal cells per total neuronal cells and the proportion of nNOS immunoreactive area of MP also decreased in aged rats. In addition, the mRNA and protein expression of c-Kit and nNOS significantly decreased with age. Expression of stem cell factor (SCF) and the pan-neuronal marker PGP 9.5 mRNA was significantly lower in the older rats than in the younger rats. Barostat studies showed no difference depending on age. Instead, the change of volume was significantly decreased by L-NG63-nitroarginine methyl ester in the 2-yr-old rats compared with the 6-wk-old rats (P = 0.003). Taken together, the quantitative and molecular nNOS changes in the stomach might play a role in the decrease of gastric accommodation with age.http://europepmc.org/articles/PMC5207530?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Yong Hwan Kwon
Nayoung Kim
Ryoung Hee Nam
Ji Hyun Park
Sun Min Lee
Sung Kook Kim
Hye Seung Lee
Yong Sung Kim
Dong Ho Lee
spellingShingle Yong Hwan Kwon
Nayoung Kim
Ryoung Hee Nam
Ji Hyun Park
Sun Min Lee
Sung Kook Kim
Hye Seung Lee
Yong Sung Kim
Dong Ho Lee
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
PLoS ONE
author_facet Yong Hwan Kwon
Nayoung Kim
Ryoung Hee Nam
Ji Hyun Park
Sun Min Lee
Sung Kook Kim
Hye Seung Lee
Yong Sung Kim
Dong Ho Lee
author_sort Yong Hwan Kwon
title Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
title_short Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
title_full Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
title_fullStr Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
title_full_unstemmed Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
title_sort change in the interstitial cells of cajal and nnos positive neuronal cells with aging in the stomach of f344 rats.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry. The density of the nNOS-positive immunoreactive area also decreased with age in the SMB, circular muscle (CM), and MP. Similarly, the percent of nNOS-positive neuronal cells per total neuronal cells and the proportion of nNOS immunoreactive area of MP also decreased in aged rats. In addition, the mRNA and protein expression of c-Kit and nNOS significantly decreased with age. Expression of stem cell factor (SCF) and the pan-neuronal marker PGP 9.5 mRNA was significantly lower in the older rats than in the younger rats. Barostat studies showed no difference depending on age. Instead, the change of volume was significantly decreased by L-NG63-nitroarginine methyl ester in the 2-yr-old rats compared with the 6-wk-old rats (P = 0.003). Taken together, the quantitative and molecular nNOS changes in the stomach might play a role in the decrease of gastric accommodation with age.
url http://europepmc.org/articles/PMC5207530?pdf=render
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