Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.
The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxid...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5207530?pdf=render |
id |
doaj-fc3d59dbf2d44e8eafbd942cf3016376 |
---|---|
record_format |
Article |
spelling |
doaj-fc3d59dbf2d44e8eafbd942cf30163762020-11-25T00:08:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016911310.1371/journal.pone.0169113Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats.Yong Hwan KwonNayoung KimRyoung Hee NamJi Hyun ParkSun Min LeeSung Kook KimHye Seung LeeYong Sung KimDong Ho LeeThe gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry. The density of the nNOS-positive immunoreactive area also decreased with age in the SMB, circular muscle (CM), and MP. Similarly, the percent of nNOS-positive neuronal cells per total neuronal cells and the proportion of nNOS immunoreactive area of MP also decreased in aged rats. In addition, the mRNA and protein expression of c-Kit and nNOS significantly decreased with age. Expression of stem cell factor (SCF) and the pan-neuronal marker PGP 9.5 mRNA was significantly lower in the older rats than in the younger rats. Barostat studies showed no difference depending on age. Instead, the change of volume was significantly decreased by L-NG63-nitroarginine methyl ester in the 2-yr-old rats compared with the 6-wk-old rats (P = 0.003). Taken together, the quantitative and molecular nNOS changes in the stomach might play a role in the decrease of gastric accommodation with age.http://europepmc.org/articles/PMC5207530?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Hwan Kwon Nayoung Kim Ryoung Hee Nam Ji Hyun Park Sun Min Lee Sung Kook Kim Hye Seung Lee Yong Sung Kim Dong Ho Lee |
spellingShingle |
Yong Hwan Kwon Nayoung Kim Ryoung Hee Nam Ji Hyun Park Sun Min Lee Sung Kook Kim Hye Seung Lee Yong Sung Kim Dong Ho Lee Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. PLoS ONE |
author_facet |
Yong Hwan Kwon Nayoung Kim Ryoung Hee Nam Ji Hyun Park Sun Min Lee Sung Kook Kim Hye Seung Lee Yong Sung Kim Dong Ho Lee |
author_sort |
Yong Hwan Kwon |
title |
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. |
title_short |
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. |
title_full |
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. |
title_fullStr |
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. |
title_full_unstemmed |
Change in the Interstitial Cells of Cajal and nNOS Positive Neuronal Cells with Aging in the Stomach of F344 Rats. |
title_sort |
change in the interstitial cells of cajal and nnos positive neuronal cells with aging in the stomach of f344 rats. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
The gastric accommodation reflex is an important mechanism in gastric physiology. However, the aging-associated structural and functional changes in gastric relaxation have not yet been established. Thus, we evaluated the molecular changes of interstitial cell of Cajal (ICC) and neuronal nitric oxide synthase (nNOS) and the function changes in the corpus of F344 rats at different ages (6-, 31-, 74-wk and 2-yr). The proportion of the c-Kit-positive area in the submucosal border (SMB) and myenteric plexus (MP) layer was significantly lower in the older rats, as indicated by immunohistochemistry. The density of the nNOS-positive immunoreactive area also decreased with age in the SMB, circular muscle (CM), and MP. Similarly, the percent of nNOS-positive neuronal cells per total neuronal cells and the proportion of nNOS immunoreactive area of MP also decreased in aged rats. In addition, the mRNA and protein expression of c-Kit and nNOS significantly decreased with age. Expression of stem cell factor (SCF) and the pan-neuronal marker PGP 9.5 mRNA was significantly lower in the older rats than in the younger rats. Barostat studies showed no difference depending on age. Instead, the change of volume was significantly decreased by L-NG63-nitroarginine methyl ester in the 2-yr-old rats compared with the 6-wk-old rats (P = 0.003). Taken together, the quantitative and molecular nNOS changes in the stomach might play a role in the decrease of gastric accommodation with age. |
url |
http://europepmc.org/articles/PMC5207530?pdf=render |
work_keys_str_mv |
AT yonghwankwon changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT nayoungkim changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT ryoungheenam changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT jihyunpark changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT sunminlee changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT sungkookkim changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT hyeseunglee changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT yongsungkim changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats AT dongholee changeintheinterstitialcellsofcajalandnnospositiveneuronalcellswithaginginthestomachoff344rats |
_version_ |
1725417153966899200 |