Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

• Main Authors: Sangmin Lee, Hojin Kim, Joonwon Kim
• Format: Article
• Language: English
• Published: SpringerOpen 2017-11-01
• Series: Micro and Nano Systems Letters
• Subjects: Pneumatic dispensing; Living cell; Viability; Proliferation
• Online Access: http://link.springer.com/article/10.1186/s40486-017-0061-y

Abstract This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials. Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation. Based on the results of a live-dead assay, more than 80% of cell viability has been confirmed which was reasonably similar to that in the control group. Furthermore, measurement of cell metabolic activity after dispensing confirmed that the dispensed cell proliferated at a rate comparable to that of the control group. These results demonstrate that the pneumatic dispensing system is a promising tool for fabrication of biomaterials.