Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge

The petroleum industry is one of the most pivotal sectors of the global economy but with it comes the production of atrocious amounts of waste especially during production and refining of the oil. Oil sludge which is an example of such waste has been categorized as hazardous because it is composed o...

Full description

Bibliographic Details
Main Authors: Brian Gidudu, Emmanuel Mudenda, Evans M.N. Chirwa
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2020-04-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/10871
id doaj-fc34855a5e284ee192faf6b5ec2b9c1c
record_format Article
spelling doaj-fc34855a5e284ee192faf6b5ec2b9c1c2021-02-16T11:33:18ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162020-04-017910.3303/CET2079073Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil SludgeBrian GiduduEmmanuel MudendaEvans M.N. ChirwaThe petroleum industry is one of the most pivotal sectors of the global economy but with it comes the production of atrocious amounts of waste especially during production and refining of the oil. Oil sludge which is an example of such waste has been categorized as hazardous because it is composed of asphaltenes, heavy metals and heavy hydrocarbons such as long-chain paraffins among others. The bioremediation of hydrocarbons in this waste is limited by low availability; biosurfactants were therefore applied to evaluate their efficacy in promoting the bioremediation process. The biosurfactants were produced by a hydrocarbon-degrading bacterium identified by 16S ribosomal RNA as Serrati sp. Biosurfactants were applied to the batch reactors containing oil sludge in different concentrations of 7 g/L, 14 g/L, and 28 g/L. The biosurfactants enhanced oil sludge biodegradation by 23.38%, 70.28%, and 55.6% respectively in 14 days. The increase in biosurfactant concentration showed a subsequent increase in microbial growth after every biosurfactant amendment due to the increase in bioavailability of the pollutants to the microbes which is utilized as the carbon and energy source. The biosurfactants were characterized by Fourier-transform infrared spectroscopy and Thin Layer chromatography showing the presence of amino acids. The biosurfactant produced by the hydrocarbon-degrading bacteria of Serratia sp. SA1 showed great potential in improving the bioremediation of recalcitrant hydrocarbons in the oil sludge and can, therefore, be used as an alternative to chemical surfactants.https://www.cetjournal.it/index.php/cet/article/view/10871
collection DOAJ
language English
format Article
sources DOAJ
author Brian Gidudu
Emmanuel Mudenda
Evans M.N. Chirwa
spellingShingle Brian Gidudu
Emmanuel Mudenda
Evans M.N. Chirwa
Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
Chemical Engineering Transactions
author_facet Brian Gidudu
Emmanuel Mudenda
Evans M.N. Chirwa
author_sort Brian Gidudu
title Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
title_short Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
title_full Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
title_fullStr Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
title_full_unstemmed Biosurfactant Produced by Serrati Sp. and Its Application in Bioremediation Enhancement of Oil Sludge
title_sort biosurfactant produced by serrati sp. and its application in bioremediation enhancement of oil sludge
publisher AIDIC Servizi S.r.l.
series Chemical Engineering Transactions
issn 2283-9216
publishDate 2020-04-01
description The petroleum industry is one of the most pivotal sectors of the global economy but with it comes the production of atrocious amounts of waste especially during production and refining of the oil. Oil sludge which is an example of such waste has been categorized as hazardous because it is composed of asphaltenes, heavy metals and heavy hydrocarbons such as long-chain paraffins among others. The bioremediation of hydrocarbons in this waste is limited by low availability; biosurfactants were therefore applied to evaluate their efficacy in promoting the bioremediation process. The biosurfactants were produced by a hydrocarbon-degrading bacterium identified by 16S ribosomal RNA as Serrati sp. Biosurfactants were applied to the batch reactors containing oil sludge in different concentrations of 7 g/L, 14 g/L, and 28 g/L. The biosurfactants enhanced oil sludge biodegradation by 23.38%, 70.28%, and 55.6% respectively in 14 days. The increase in biosurfactant concentration showed a subsequent increase in microbial growth after every biosurfactant amendment due to the increase in bioavailability of the pollutants to the microbes which is utilized as the carbon and energy source. The biosurfactants were characterized by Fourier-transform infrared spectroscopy and Thin Layer chromatography showing the presence of amino acids. The biosurfactant produced by the hydrocarbon-degrading bacteria of Serratia sp. SA1 showed great potential in improving the bioremediation of recalcitrant hydrocarbons in the oil sludge and can, therefore, be used as an alternative to chemical surfactants.
url https://www.cetjournal.it/index.php/cet/article/view/10871
work_keys_str_mv AT briangidudu biosurfactantproducedbyserratispanditsapplicationinbioremediationenhancementofoilsludge
AT emmanuelmudenda biosurfactantproducedbyserratispanditsapplicationinbioremediationenhancementofoilsludge
AT evansmnchirwa biosurfactantproducedbyserratispanditsapplicationinbioremediationenhancementofoilsludge
_version_ 1724267742203215872