Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress

Bacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resis...

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Main Authors: Constance Porrini, Cyprien Guérin, Seav-Ly Tran, Rozenn Dervyn, Pierre Nicolas, Nalini Ramarao
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/22/10/5079
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spelling doaj-fc18cca0439b4f59bd4d54420c7322722021-05-31T23:42:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225079507910.3390/ijms22105079Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide StressConstance Porrini0Cyprien Guérin1Seav-Ly Tran2Rozenn Dervyn3Pierre Nicolas4Nalini Ramarao5Micalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMaIAGE, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMaIAGE, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, AgroParisTech, INRAE, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceBacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resistance by Gram-positive bacteria are poorly described. In the opportunistic foodborne pathogen <i>Bacillus cereus</i>, genome sequence analyses did not identify homologs to known NO reductases and transcriptional regulators, such as NsrR, which orchestrate the response to NO of other pathogenic or non-pathogenic bacteria. Using a transcriptomic approach, we investigated the adaptation of <i>B. cereus</i> to NO stress. A cluster of 6 genes was identified to be strongly up-regulated in the early phase of the response. This cluster contains an iron-sulfur cluster repair enzyme, a nitrite reductase and three enzymes involved in siroheme biosynthesis. The expression pattern and close genetic localization suggest a functional link between these genes, which may play a pivotal role in the resistance of <i>B. cereus</i> to NO stress during infection.https://www.mdpi.com/1422-0067/22/10/5079<i>Bacillus cereus</i>nitric oxideiron cluster repairtranscriptomic
collection DOAJ
language English
format Article
sources DOAJ
author Constance Porrini
Cyprien Guérin
Seav-Ly Tran
Rozenn Dervyn
Pierre Nicolas
Nalini Ramarao
spellingShingle Constance Porrini
Cyprien Guérin
Seav-Ly Tran
Rozenn Dervyn
Pierre Nicolas
Nalini Ramarao
Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
International Journal of Molecular Sciences
<i>Bacillus cereus</i>
nitric oxide
iron cluster repair
transcriptomic
author_facet Constance Porrini
Cyprien Guérin
Seav-Ly Tran
Rozenn Dervyn
Pierre Nicolas
Nalini Ramarao
author_sort Constance Porrini
title Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
title_short Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
title_full Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
title_fullStr Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
title_full_unstemmed Implication of a Key Region of Six <i>Bacillus cereus</i> Genes Involved in Siroheme Synthesis, Nitrite Reductase Production and Iron Cluster Repair in the Bacterial Response to Nitric Oxide Stress
title_sort implication of a key region of six <i>bacillus cereus</i> genes involved in siroheme synthesis, nitrite reductase production and iron cluster repair in the bacterial response to nitric oxide stress
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-05-01
description Bacterial response to nitric oxide (NO) is of major importance for bacterial survival. NO stress is a main actor of the eukaryotic immune response and several pathogenic bacteria have developed means for detoxification and repair of the damages caused by NO. However, bacterial mechanisms of NO resistance by Gram-positive bacteria are poorly described. In the opportunistic foodborne pathogen <i>Bacillus cereus</i>, genome sequence analyses did not identify homologs to known NO reductases and transcriptional regulators, such as NsrR, which orchestrate the response to NO of other pathogenic or non-pathogenic bacteria. Using a transcriptomic approach, we investigated the adaptation of <i>B. cereus</i> to NO stress. A cluster of 6 genes was identified to be strongly up-regulated in the early phase of the response. This cluster contains an iron-sulfur cluster repair enzyme, a nitrite reductase and three enzymes involved in siroheme biosynthesis. The expression pattern and close genetic localization suggest a functional link between these genes, which may play a pivotal role in the resistance of <i>B. cereus</i> to NO stress during infection.
topic <i>Bacillus cereus</i>
nitric oxide
iron cluster repair
transcriptomic
url https://www.mdpi.com/1422-0067/22/10/5079
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