<i>Pichia pastoris</i> and the Recombinant Human Heterodimeric Amino Acid Transporter 4F2hc-LAT1: From Clone Selection to Pure Protein

Heterodimeric amino acid transporters (HATs) are protein complexes composed of two subunits, a heavy and a light subunit belonging to the solute carrier (SLC) families SLC3 and SLC7. HATs transport amino acids and derivatives thereof across the plasma membrane. The human HAT 4F2hc-LAT1 is composed o...

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Bibliographic Details
Main Authors: Satish Kantipudi, Daniel Harder, Sara Bonetti, Dimitrios Fotiadis, Jean-Marc Jeckelmann
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Methods and Protocols
Subjects:
Online Access:https://www.mdpi.com/2409-9279/4/3/51