Optimization of Quantitative PCR Methods for Enteropathogen Detection.

Optimization of Quantitative PCR Methods for Enteropathogen Detection.

Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficien...

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Main Authors: Jie Liu, Jean Gratz, Caroline Amour, Rosemary Nshama, Thomas Walongo, Athanasia Maro, Esto Mduma, James Platts-Mills, Nadia Boisen, James Nataro, Doris M Haverstick, Furqan Kabir, Paphavee Lertsethtakarn, Sasikorn Silapong, Pimmada Jeamwattanalert, Ladaporn Bodhidatta, Carl Mason, Sharmin Begum, Rashidul Haque, Ira Praharaj, Gagandeep Kang, Eric R Houpt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4918952?pdf=render

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