Validation of a yeast malate dehydrogenase 2 (Mdh2) antibody tested for use in western blots [version 2; referees: 2 approved]

• Main Authors: Shiran Gabay-Maskit, Maya Schuldiner, Einat Zalckvar
• Format: Article
• Language: English
• Published: F1000 Research Ltd 2018-07-01
• Series: F1000Research
• Subjects: Cell Signaling
• Online Access: https://f1000research.com/articles/7-130/v2

Malate dehydrogenases (Mdhs) reversibly convert malate to oxaloacetate and serve as important enzymes in several metabolic pathways. In the yeast Saccharomyces cerevisiae there are three Mdh isozymes, localized to different compartments in the cell. In order to identify specifically the Mdh2 isozyme, GenScript USA produced three different antibodies that we further tested by western blot. All three antibodies recognized the S. cerevisiae Mdh2 with different background and specificity properties. One of the antibodies had a relatively low background and high specificity and thus can be used for specific identification of Mdh2 in various experimental settings.