Biochar-carrying hydrocarbon decomposers promote degradation during the early stage of bioremediation
Oil pollution is one of the most serious current environmental problems. In this study, four strategies of bioremediation of oil-polluted soil were tested in the laboratory over a period of 84 days: (A) aeration and moistening; (B) amendment with 1 % biochar (<i>w</i> &fra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2016-10-01
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| Series: | Biogeosciences |
| Online Access: | http://www.biogeosciences.net/13/5739/2016/bg-13-5739-2016.pdf |
| Summary: | Oil pollution is one of the most serious current environmental problems. In
this study, four strategies of bioremediation of oil-polluted soil were
tested in the laboratory over a period of 84 days: (A) aeration and
moistening; (B) amendment with 1 % biochar (<i>w</i> ⁄ <i>w</i>) in combination with A;
amendment with 1 % biochar with immobilized <i>Pseudomonas aeruginosa</i>
(C) or <i>Acinetobacter radioresistens</i> (D) in combination with A. All
strategies used resulted in a decrease of the hydrocarbon content, while
biochar addition (B, C, D strategies) led to acceleration of decomposition in
the beginning. Microbial biomass and respiration rate increased significantly
at the start of bioremediation. It was demonstrated that moistening and
aeration were the main factors influencing microbial biomass, while
implementation of biochar and introduction of microbes were the main factors
influencing microbial respiration. All four remediation strategies altered
bacterial community structure and phytotoxicity. The Illumina MiSeq method
revealed 391 unique operational taxonomic units (OTUs) belonging to 40 bacterial phyla and a domination of
<i>Proteobacteria</i> in all investigated soil samples. The lowest
alpha diversity was observed in the samples with introduced bacteria on the
first day of remediation. Metric multidimensional scaling demonstrated that
in the beginning and at the end, microbial community structures were more
similar than those on the 28th day of remediation. Strategies A and B
decreased phytotoxicity of remediated soil between 2.5 and 3.1 times as
compared with untreated soil. C and D strategies led to additional decrease
of phytotoxicity between 2.1 and 3.2 times. |
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| ISSN: | 1726-4170 1726-4189 |