Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects

Abstract Modern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work po...

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Bibliographic Details
Main Authors: Alexey A. Polilov, Anastasia A. Makarova, Song Pang, C. Shan Xu, Harald Hess
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-83936-0