A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro

• Main Authors: G. Baillie, M.D. Owens, G.W. Halbert
• Format: Article
• Language: English
• Published: Elsevier 2002-01-01
• Series: Journal of Lipid Research
• Subjects: U937; low density lipoprotein; amphipathic peptide; apoB; synthetic LDL; phosphate-buffered saline
• Online Access: http://www.sciencedirect.com/science/article/pii/S0022227520301887

A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application. —Baillie, G., M. D. Owens, and G. W. Halbert. A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro. J. Lipid Res. 2002. 43: 69–73.