The influence of DNA polymorphism of multidrug resistant 1 (MDR1) on the effect of midazolam pretreatment in children

• Main Authors: Hyo-Jin Byon, Keun-Suk Park, Yong-Hee Park, Jin-Tae Kim, Chul-Woo Jung, Hee-Soo Kim
• Format: Article
• Language: English
• Published: Korean Society of Anesthesiologists 2012-04-01
• Series: Korean Journal of Anesthesiology
• Subjects: genetic polymorphism; mdr1; midazolam
• Online Access: http://ekja.org/upload/pdf/kjae-62-332.pdf

BackgroundPatients showed a different response following intravenous midazolam injection. Some children showed irritability or were not sedated by midazolam. We hypothesized that there may be genetic variations of the MDR1 gene, based on the response to midazolam.MethodsOne hundred and ninety-three pediatric patients were recruited in this study. Midazolam (0.1 mg/kg) was injected intravenously before surgery. Anxiety score (activity, vocalizations, emotional expressivity, state of apparent arousal) was checked before and 5 minutes after midazolam injection. In addition, other manifestations after midazolam injection were recorded. After anesthesia, 2 ml of blood was sampled. Children were genotyped MDR1. Haplotype was analyzed using the software package PHASE, version 2.0.ResultsThe observed frequencies of MDR1 haplotype of TTT, TGC, CAC, CGC were 0.334, 0.205, 0.182 and 0.225, respectively. There was no significant correlation between the response of midazolam and the MDR1 haplotype of TTT, TGC, CAC or CGC (P = 0.98).ConclusionsGenotyping of MDR1 may not be related to the response of midazolam in children.