Expression and purification of the immunogenically active fragment B of the Park Williams 8 Corynebacterium diphtheriae strain toxin

Expression and purification of the immunogenically active fragment B of the Park Williams 8 Corynebacterium diphtheriae strain toxin

The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently availab...

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Bibliographic Details
Main Authors: D.V. Nascimento, E.M.B. Lemes, J.L.S. Queiroz, J.G. Silva Jr., H.J. Nascimento, E.D. Silva, R. Hirata Jr., A.A.S.O. Dias, C.S. Santos, G.M.B. Pereira, A.L. Mattos-Guaraldi, G.R.G. Armoa
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2010-05-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Fragment B
Diphtheria toxin
Diphtheria
dtb gene
E. coli gene expression
Immobilized metal affinity
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500007

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