Nitrogen Dioxide at Ambient Concentrations Induces Nitration and Degradation of PYR/PYL/RCAR Receptors to Stimulate Plant Growth: A Hypothetical Model

• Main Authors: Misa Takahashi, Hiromichi Morikawa
• Format: Article
• Language: English
• Published: MDPI AG 2019-06-01
• Series: Plants
• Subjects: nitrogen dioxide; <i>Arabidopsis thaliana</i>; plant growth; cell enlargement; cell proliferation; early flowering; tyrosine nitration; PsbO
• Online Access: https://www.mdpi.com/2223-7747/8/7/198

Exposing <i>Arabidopsis thaliana</i> (Arabidopsis) seedlings fed with soil nitrogen to 10&#8722;50 ppb nitrogen dioxide (NO₂) for several weeks stimulated the uptake of major elements, photosynthesis, and cellular metabolisms to more than double the biomass of shoot, total leaf area and contents of N, C P, K, S, Ca and Mg per shoot relative to non-exposed control seedlings. The ¹⁵N/¹⁴N ratio analysis by mass spectrometry revealed that N derived from NO₂ (NO₂-N) comprised &lt; 5% of the total plant N, showing that the contribution of NO₂-N as N source was minor. Moreover, histological analysis showed that leaf size and biomass were increased upon NO₂ treatment, and that these increases were attributable to leaf age-dependent enhancement of cell proliferation and enlargement. Thus, NO₂ may act as a plant growth signal rather than an N source. Exposure of Arabidopsis leaves to 40 ppm NO₂ induced virtually exclusive nitration of PsbO and PsbP proteins (a high concentration of NO₂ was used). The PMF analysis identified the ninth tyrosine residue of PsbO1 (⁹Tyr) as a nitration site. ⁹Tyr of PsbO1 was exclusively nitrated after incubation of the thylakoid membranes with a buffer containing NO₂ and NO₂^&#8722; or a buffer containing NO₂^&#8722; alone. Nitration was catalyzed by illumination and repressed by photosystem II (PSII) electron transport inhibitors, and decreased oxygen evolution. Thus, protein tyrosine nitration altered (downregulated) the physiological function of cellular proteins of Arabidopsis leaves. This indicates that NO₂-induced protein tyrosine nitration may stimulate plant growth. We hypothesized that atmospheric NO₂ at ambient concentrations may induce tyrosine nitration of PYR/PYL/RCAR receptors in Arabidopsis leaves, followed by degradation of PYR/PYL/RCAR, upregulation of target of rapamycin (TOR) regulatory complexes, and stimulation of plant growth.