Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology

This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay qu...

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Bibliographic Details
Main Authors: Goldin John, Nikhil Shri Sahajpal, Ashis K. Mondal, Sudha Ananth, Colin Williams, Alka Chaubey, Amyn M. Rojiani, Ravindra Kolhe
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Current Issues in Molecular Biology
Subjects:
next-generation sequencing
COVID-19
challenges
diagnostic assay
Online Access:https://www.mdpi.com/1467-3045/43/2/61
Description
Summary:This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
ISSN:1467-3045

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