Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota

Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota

Abstract Background Low-biomass microbiome studies (such as those of the lungs, placenta, and skin) are vulnerable to contamination and sequencing stochasticity, which obscure legitimate microbial signal. While human lung microbiome studies have rigorously identified sampling strategies that reliabl...

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Bibliographic Details
Main Authors: Jennifer M. Baker, Kevin J. Hinkle, Roderick A. McDonald, Christopher A. Brown, Nicole R. Falkowski, Gary B. Huffnagle, Robert P. Dickson
Format: Article
Language:English
Published: BMC 2021-05-01
Series:Microbiome
Subjects:
Lung microbiome
16S rRNA gene amplicon sequencing
Bronchoalveolar lavage
Whole lung tissue
Online Access:https://doi.org/10.1186/s40168-021-01055-4

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