Hesperidin triggering apoptosis on neuroblastoma cell

• Main Authors: Tolga Çatmakaş, Barış Ertuğrul, Elif Sinem İplik, Bedia Çakmakoğlu
• Format: Article
• Language: English
• Published: Archives of Clinical and Experimental Medicine 2018-11-01
• Series: Archives of Clinical and Experimental Medicine
• Subjects: nöroblastoma; hesperidin; apoptoz; Hesperidin; neuroblastoma; apoptosis
• Online Access: http://dergipark.org.tr/acem/issue/39803/449087?publisher=www-hasbahceci-com

Aim: Neuroblastoma accounts for approximately %10 of all pediatric cancers and approximately %15 of cancer related deaths in children. Understanding of the molecular mechanisms which play role in the progress of this cancer type may lead to develop more effective strategies for therapy. Flavanoids are popular subject for this new strategies. Hesperidin is an herbal flavonoid which is found abundantly in citrus that has been studied on several cancer cell lines. For this purpose, it was aimed to investigate is the apoptotic effects of hesperidin on neuroblastoma tumors using SH-SY5Y cell line.Methods: Hesperidin was performed on SH-SY5Y and MRC-5 cell line by WST-1, Caspase-3 and Annexin V in a time and dose dependent manner. Results: 2.5 µM hesperidin and 5 µM hesperidin were found the most suitable dosage for neuroblastoma cell line because of the success on decreasing cell proliferation. Hesperidin has resulted with the ability for apoptotic cell death compared with control group [MRC-5 cell line, p<0.05 for all]. 2,5 µM and 5 µM hesperidin concentration for 48h were ended up early apoptotic results as 53.65% for 2.5 µM and 38.90% for 5 µM. There was no significant change on caspase-3 activity. Conclusions: Our study suggests that hesperidin would be effective against neuroblastoma tumors. We believe with further investigation this study will be helpful for developing new research areas in neuroblastoma tumors.