Antioxidant and Anti-Inflammatory Effects of <i>Zingiber officinale roscoe</i> and <i>Allium subhirsutum</i>: In Silico, Biochemical and Histological Study

• Main Authors: Nourhene Zammel, Mohd Saeed, Nouha Bouali, Salem Elkahoui, Jahoor M. Alam, Tarek Rebai, Mohd A. Kausar, Mohd Adnan, Arif J. Siddiqui, Riadh Badraoui
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Foods
• Subjects: antioxidants; inflammation; oxidative stress; <i>Zingiber officinale roscoe</i>; <i>Allium subhirsutum</i>; inflammatory biomarkers
• Online Access: https://www.mdpi.com/2304-8158/10/6/1383

In this study, the antioxidant and anti-inflammatory effects of <i>Zingiber officinale roscoe</i> and <i>Allium subhirsutum</i> aqueous extracts were examined in a carrageenan-induced acute inflammation model. Some markers of inflammation such as hematological parameters, fibrinogen and C-reactive protein were measured. Variables reflecting oxidative stress included thiobarbituric acid reactive substances (TBARS), advanced oxidation of protein products (AOPP), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione were determined in both inflamed foci and erythrocytes. The in silico molecular docking simulation showed that the main components of <i>Zingiber officinale roscoe</i> and <i>Allium subhirsutum</i> bound to toll-like receptor 6 (TLR6) with high affinities. Moreover, histological examinations of paw edema were carried out. Both <i>Zingiber officinale roscoe</i> and <i>Allium subhirsutum</i> ameliorated the induced inflammation and oxidative stress status as outlined by anti-edematous, antioxidant and anti-inflammatory activities. Our investigation lends pharmacological support to the medical uses of these spices in the management of inflammatory disorders and oxidative damage. The results of the in silico assay satisfactory explain the in vivo effects as compared with indomethacin.