Crystal structure of a complex of DNA with one AT-hook of HMGA1.

Crystal structure of a complex of DNA with one AT-hook of HMGA1.

We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is foun...

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Main Authors: Elsa Fonfría-Subirós, Francisco Acosta-Reyes, Núria Saperas, Joan Pous, Juan A Subirana, J Lourdes Campos
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3353895?pdf=render
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spelling doaj-fa0cc79827a74295aba4fd1fe3c4ad072020-11-24T21:55:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3712010.1371/journal.pone.0037120Crystal structure of a complex of DNA with one AT-hook of HMGA1.Elsa Fonfría-SubirósFrancisco Acosta-ReyesNúria SaperasJoan PousJuan A SubiranaJ Lourdes CamposWe present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is found. The structure presents analogies and significant differences with previous NMR studies: the AT-hook forms hydrogen bonds between main-chain NH groups and thymines in the minor groove, DNA is bent and the minor groove is widened.http://europepmc.org/articles/PMC3353895?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Elsa Fonfría-Subirós
Francisco Acosta-Reyes
Núria Saperas
Joan Pous
Juan A Subirana
J Lourdes Campos
spellingShingle Elsa Fonfría-Subirós
Francisco Acosta-Reyes
Núria Saperas
Joan Pous
Juan A Subirana
J Lourdes Campos
Crystal structure of a complex of DNA with one AT-hook of HMGA1.
PLoS ONE
author_facet Elsa Fonfría-Subirós
Francisco Acosta-Reyes
Núria Saperas
Joan Pous
Juan A Subirana
J Lourdes Campos
author_sort Elsa Fonfría-Subirós
title Crystal structure of a complex of DNA with one AT-hook of HMGA1.
title_short Crystal structure of a complex of DNA with one AT-hook of HMGA1.
title_full Crystal structure of a complex of DNA with one AT-hook of HMGA1.
title_fullStr Crystal structure of a complex of DNA with one AT-hook of HMGA1.
title_full_unstemmed Crystal structure of a complex of DNA with one AT-hook of HMGA1.
title_sort crystal structure of a complex of dna with one at-hook of hmga1.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description We present here for the first time the crystal structure of an AT-hook domain. We show the structure of an AT-hook of the ubiquitous nuclear protein HMGA1, combined with the oligonucleotide d(CGAATTAATTCG)(2), which has two potential AATT interacting groups. Interaction with only one of them is found. The structure presents analogies and significant differences with previous NMR studies: the AT-hook forms hydrogen bonds between main-chain NH groups and thymines in the minor groove, DNA is bent and the minor groove is widened.
url http://europepmc.org/articles/PMC3353895?pdf=render
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AT juanasubirana crystalstructureofacomplexofdnawithoneathookofhmga1
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