Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates

The study aimed to investigate the prevalence and resistance pattern of different Citrobacter spp., phenotypically and genotypically, to β-lactam antibiotics, then to evaluate the antibacterial activity of omega-3 extracted from flaxseed against isolates that harboring resistance genes. Herein, 19 C...

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Main Author: Darweesh Mayyada F.
Format: Article
Language:English
Published: Sciendo 2017-09-01
Series:Current Issues in Pharmacy and Medical Sciences
Subjects:
Online Access:https://doi.org/10.1515/cipms-2017-0029
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spelling doaj-fa0435541aee43e1ac68c0013fed9e162021-09-06T19:19:39ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762017-09-0130315616110.1515/cipms-2017-0029cipms-2017-0029Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolatesDarweesh Mayyada F.0Biology Department, Faculty of Science, Kufa University, Najaf, IraqThe study aimed to investigate the prevalence and resistance pattern of different Citrobacter spp., phenotypically and genotypically, to β-lactam antibiotics, then to evaluate the antibacterial activity of omega-3 extracted from flaxseed against isolates that harboring resistance genes. Herein, 19 Citrobacter isolates were isolated from 100 stool and urine samples taken from patients attended to AL-Sadar Hospital during June- December 2016. Clinical samples were then cultured on specific media, thereafter, isolates were identified depending on morphological, biochemical characteristics and VITK-2. The results showed that the Citrobacter spp. comprise 19/78 (24%) of positive bacterial growth on macConky agar, from which 14.1% were C. freundii, C. koseri represented 6.1% and C. farmeri were 3.8% of the total. The results of antibiotic susceptibility showed that all Citrobacter 100% isolates were resistant to ampicillin and cefoxitin, but were sensitive to imipinim. Moreover, the isolates initally showed different degrees of resistant to β-lactam antibiotics. Furthermore, by confirmatory test, the results observed that 17/19 (89.4%) of the isolated were extended-spectrum β-lactamase (ESBL - producers). Finally, using the PCR technique to detect blaGenes, the results revealed that 14/17 (82.3) of potential ESBL producing Citrobacter harbored one or more ESBL genes. These included 10 isolates of C. freundii and 4 isolates of C. koseri. In related work, extracts of essential fatty acid semicarbazide - omega3 (EFASC) from Linum usitatissium (Flaxseed) were tested to evaluate their activity against resistant isolates. The results demonstrate the broad spectrum antibacterial property of EFASC compounds against resistant bacteria. In conclusion, this study found increase prevalence of multi-drug resistance MDR Citrobacter spp. as causative agents in clinical cases. Considering the antibacterial activity of EFASC that displayed high activity against resistant pathogens, deservedly, attention must be paid to developing their use as alternative antibiotics.https://doi.org/10.1515/cipms-2017-0029citrobacter spp.esbl-genesomega-3 antibacterial activity
collection DOAJ
language English
format Article
sources DOAJ
author Darweesh Mayyada F.
spellingShingle Darweesh Mayyada F.
Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
Current Issues in Pharmacy and Medical Sciences
citrobacter spp.
esbl-genes
omega-3 antibacterial activity
author_facet Darweesh Mayyada F.
author_sort Darweesh Mayyada F.
title Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
title_short Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
title_full Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
title_fullStr Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
title_full_unstemmed Molecular characterization of ESBL gene in Citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
title_sort molecular characterization of esbl gene in citrobacter spp. and antibacterial activity of omega-3 against resistant isolates
publisher Sciendo
series Current Issues in Pharmacy and Medical Sciences
issn 2084-980X
2300-6676
publishDate 2017-09-01
description The study aimed to investigate the prevalence and resistance pattern of different Citrobacter spp., phenotypically and genotypically, to β-lactam antibiotics, then to evaluate the antibacterial activity of omega-3 extracted from flaxseed against isolates that harboring resistance genes. Herein, 19 Citrobacter isolates were isolated from 100 stool and urine samples taken from patients attended to AL-Sadar Hospital during June- December 2016. Clinical samples were then cultured on specific media, thereafter, isolates were identified depending on morphological, biochemical characteristics and VITK-2. The results showed that the Citrobacter spp. comprise 19/78 (24%) of positive bacterial growth on macConky agar, from which 14.1% were C. freundii, C. koseri represented 6.1% and C. farmeri were 3.8% of the total. The results of antibiotic susceptibility showed that all Citrobacter 100% isolates were resistant to ampicillin and cefoxitin, but were sensitive to imipinim. Moreover, the isolates initally showed different degrees of resistant to β-lactam antibiotics. Furthermore, by confirmatory test, the results observed that 17/19 (89.4%) of the isolated were extended-spectrum β-lactamase (ESBL - producers). Finally, using the PCR technique to detect blaGenes, the results revealed that 14/17 (82.3) of potential ESBL producing Citrobacter harbored one or more ESBL genes. These included 10 isolates of C. freundii and 4 isolates of C. koseri. In related work, extracts of essential fatty acid semicarbazide - omega3 (EFASC) from Linum usitatissium (Flaxseed) were tested to evaluate their activity against resistant isolates. The results demonstrate the broad spectrum antibacterial property of EFASC compounds against resistant bacteria. In conclusion, this study found increase prevalence of multi-drug resistance MDR Citrobacter spp. as causative agents in clinical cases. Considering the antibacterial activity of EFASC that displayed high activity against resistant pathogens, deservedly, attention must be paid to developing their use as alternative antibiotics.
topic citrobacter spp.
esbl-genes
omega-3 antibacterial activity
url https://doi.org/10.1515/cipms-2017-0029
work_keys_str_mv AT darweeshmayyadaf molecularcharacterizationofesblgeneincitrobactersppandantibacterialactivityofomega3againstresistantisolates
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