Object-Based Analyses in FIJI/ImageJ to Measure Local RNA Translation Sites in Neurites in Response to Aβ1-42 Oligomers

Object-Based Analyses in FIJI/ImageJ to Measure Local RNA Translation Sites in Neurites in Response to Aβ1-42 Oligomers

Subcellular protein delivery is especially important in signal transduction and cell behavior, and is typically achieved by localization signals within the protein. However, protein delivery can also rely on localization of mRNAs that are translated at target sites. Although once considered heretica...

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Bibliographic Details
Main Authors: María Gamarra, Maite Blanco-Urrejola, Andreia F. R. Batista, Josune Imaz, Jimena Baleriola
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Neuroscience
Subjects:
local protein synthesis
RNA localization
neurites
fluorescence microscopy
FIJI/ImageJ analyses
colocalization analyses
Online Access:https://www.frontiersin.org/article/10.3389/fnins.2020.00547/full

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