Anti Tuberculosis Activity of Forest Kedondong (Spondias pinnata) Stembark Extract Against Multiple Drug Resistance (MDR) Strain of Mycobacterium Tuberculosis
<p><strong>Background:</strong> Forest Kedondong (<em>Spondias pinnata</em>) traditionally known as “<em>loloh cemcem</em>” and commonly used as a chronic cough remedy. Previous research showed that methanol extract of Forest Kedondong leaves active against...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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DiscoverSys
2016-04-01
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| Series: | Bali Medical Journal |
| Subjects: | |
| Online Access: | https://balimedicaljournal.org/index.php/bmj/article/view/190 |
| Summary: | <p><strong>Background:</strong> Forest Kedondong (<em>Spondias pinnata</em>) traditionally known as “<em>loloh cemcem</em>” and commonly used as a chronic cough remedy. Previous research showed that methanol extract of Forest Kedondong leaves active against MDR strain of <em>Mycobacterium tuberculosis</em>. The aim of this study were to determine the phytochemical constituent and anti tuberculosis activity of stem bark extract of this plant against MDR strain of <em>M. tuberculosis</em>. <strong>Method:</strong> Coarsely powder of Forest Kedondong stem bark was extracted successively with n-hexane, chloroform and 80% ethanol. Anti tuberculosis assay of chloroform and ethanol extract was conducted using proportion method with Lowenstein-Jensen medium within 3 different concentration of extract of 1, 10, and 100 mg/mL. Activity was evaluated by inhibition of extract against <em>M. tuberculosis </em>growth, which was calculated by mean reduction in number of colonies on extract containing medium compared to control. <strong>Results and Discussion:</strong> Phytochemical test showed that chloroform extract contains terpenoid and flavonoids. Ethanol extract contains terpenoid, polyphenols and flavonoids. These extracts were active against MDR strain of <em>M. tuberculosis</em> with 100% inhibition at concentration of 100 mg/mL. Chloroform extract has higher inhibition against <em>M. tuberculosis</em> growth than Ethanol extract. <strong>Conclusions:</strong> These extracts were potentially developed to an anti tuberculosis constituent from natural product.</p> |
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| ISSN: | 2089-1180 2302-2914 |