Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk
The prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are oft...
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Taylor & Francis Group
2019-12-01
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| Series: | Journal of Extracellular Vesicles |
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| Online Access: | http://dx.doi.org/10.1080/20013078.2019.1578116 |
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doaj-f992b281462943199be76c0d04f1d8692020-11-25T01:34:23ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782019-12-018110.1080/20013078.2019.15781161578116Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talkEline P. Hansen0Bastian Fromm1Sidsel D. Andersen2Antonio Marcilla3Kasper L. Andersen4Anne Borup5Andrew R. Williams6Aaron R. Jex7Robin B. Gasser8Neil D. Young9Ross S. Hall10Allan Stensballe11Vladimir Ovchinnikov12Yan Yan13Merete Fredholm14Stig M. Thamsborg15Peter Nejsum16University of CopenhagenOslo University HospitalAarhus UniversityUniversitat de ValénciaUniversity of CopenhagenAarhus UniversityUniversity of CopenhagenThe Walter and Eliza Hall InstituteThe Walter and Eliza Hall InstituteThe Walter and Eliza Hall InstituteThe Walter and Eliza Hall InstituteAalborg UniversityThe Siberian Branch of the Russian Academy of SciencesAarhus UniversityUniversity of CopenhagenUniversity of CopenhagenAarhus UniversityThe prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are often chronic in nature, and the parasites have a profound ability to modulate their hosts’ immune responses. This study provides the first in-depth characterisation of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and proposes the role of these vesicles in the host–parasite interplay. The release of EVs from the third- (L3) and fourth-stage (L4) larvae and adults was demonstrated by transmission electron microscopy (TEM), and sequencing of EV-derived RNA identified a number of microRNAs (miRNAs) and transcripts of potential host immune targets, such as IL-13, IL-25 and IL-33, were identified. Furthermore, proteomics of EVs identified several proteins with immunomodulatory properties and other proteins previously shown to be associated with parasite EVs. Taken together, these results suggest that A. suum EVs and their cargo may play a role in host–parasite interactions. This knowledge may pave the way to novel strategies for helminth infection control and knowledge of their immune modulatory potential.http://dx.doi.org/10.1080/20013078.2019.1578116ascaris suumhost–parasite interactionsextracellular vesiclesimmunitymirnaproteomics |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Eline P. Hansen Bastian Fromm Sidsel D. Andersen Antonio Marcilla Kasper L. Andersen Anne Borup Andrew R. Williams Aaron R. Jex Robin B. Gasser Neil D. Young Ross S. Hall Allan Stensballe Vladimir Ovchinnikov Yan Yan Merete Fredholm Stig M. Thamsborg Peter Nejsum |
| spellingShingle |
Eline P. Hansen Bastian Fromm Sidsel D. Andersen Antonio Marcilla Kasper L. Andersen Anne Borup Andrew R. Williams Aaron R. Jex Robin B. Gasser Neil D. Young Ross S. Hall Allan Stensballe Vladimir Ovchinnikov Yan Yan Merete Fredholm Stig M. Thamsborg Peter Nejsum Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk Journal of Extracellular Vesicles ascaris suum host–parasite interactions extracellular vesicles immunity mirna proteomics |
| author_facet |
Eline P. Hansen Bastian Fromm Sidsel D. Andersen Antonio Marcilla Kasper L. Andersen Anne Borup Andrew R. Williams Aaron R. Jex Robin B. Gasser Neil D. Young Ross S. Hall Allan Stensballe Vladimir Ovchinnikov Yan Yan Merete Fredholm Stig M. Thamsborg Peter Nejsum |
| author_sort |
Eline P. Hansen |
| title |
Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk |
| title_short |
Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk |
| title_full |
Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk |
| title_fullStr |
Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk |
| title_full_unstemmed |
Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite–host cross talk |
| title_sort |
exploration of extracellular vesicles from ascaris suum provides evidence of parasite–host cross talk |
| publisher |
Taylor & Francis Group |
| series |
Journal of Extracellular Vesicles |
| issn |
2001-3078 |
| publishDate |
2019-12-01 |
| description |
The prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are often chronic in nature, and the parasites have a profound ability to modulate their hosts’ immune responses. This study provides the first in-depth characterisation of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and proposes the role of these vesicles in the host–parasite interplay. The release of EVs from the third- (L3) and fourth-stage (L4) larvae and adults was demonstrated by transmission electron microscopy (TEM), and sequencing of EV-derived RNA identified a number of microRNAs (miRNAs) and transcripts of potential host immune targets, such as IL-13, IL-25 and IL-33, were identified. Furthermore, proteomics of EVs identified several proteins with immunomodulatory properties and other proteins previously shown to be associated with parasite EVs. Taken together, these results suggest that A. suum EVs and their cargo may play a role in host–parasite interactions. This knowledge may pave the way to novel strategies for helminth infection control and knowledge of their immune modulatory potential. |
| topic |
ascaris suum host–parasite interactions extracellular vesicles immunity mirna proteomics |
| url |
http://dx.doi.org/10.1080/20013078.2019.1578116 |
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