Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions

• Main Authors: Milosavić Nenad B., Prodanović Radivoje M., Jovanović Slobodan M., Maksimović Vuk M., Vujčić Zoran M.
• Format: Article
• Language: English
• Published: Association of Chemical Engineers of Serbia 2004-01-01
• Series: Hemijska Industrija
• Subjects: glucoamylase; enzyme reactor; periodate; poly (GMA-co-EGDMA); immobilization; stability
• Online Access: http://www.doiserbia.nb.rs/img/doi/0367-598X/2004/0367-598X0411493M.pdf
• ISSN: 0367-598X

The application of amyloglucosidase immobilized on the macroporous co-polymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) in an enzyme reactor was shown. The higher thermostability of immobilized glucoamylases than the soluble one was demonstrated. Immobilized amyloglucosidase obtained by the periodate method shows two times higher thermo stability than the soluble form. Glucoamylases immobilized on poly (GMA-co-EGDMA) have good mechanical and chemical features in the reactor and when applied in a continuous flow reactor for 28 days no changes are observed. In this period periodate immobilized amyloglucosidase shows no decrease in activity. It showed potential for the continuous production of glucose from starch over a prolonged period of time.