NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES

• Main Author: Pavlovich О. V.
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2014-02-01
• Series: Biotechnologia Acta
• Subjects: SPEV cell line; fibroblasts; nanoparticles; luminophores
• Online Access: http://biotechnology.kiev.ua/images/storage/no1_2014/pavlovich.pdf

The morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 μg/ml for SPEV cell line, human fibroblasts in suspension were investigated. The localization of Eu2O3 nanoparticles in SPEV cells and fibroblasts was determined by confocal scanning microscopy. We found that europium oxide nanoparticles at a concentration of 6.8 μg/ml did not have a toxic effect on the cell cultures, luminesce in the visible spectral range and remain for a long time in the cells without a significant decrease in intensity of the luminescence. The localization of nanoparticles in cell cytoplasme but not in nuclei was shown by confocal scanning microscopy. They can be used as nanoluminophores for labelling of cell suspensions and cultures.