Functional Similarity and Difference among <i>Bra-MIR319</i> Family in Plant Development

• Main Authors: Ziwei Hu, Tingting Liu, Jiashu Cao
• Format: Article
• Language: English
• Published: MDPI AG 2019-11-01
• Series: Genes
• Subjects: <i>brassica campestris</i>; bra-mir319 family; leaf and petal morphogenesis; pollen development
• Online Access: https://www.mdpi.com/2073-4425/10/12/952

miR319 was the first plant miRNA discovered via forward genetic mutation screening. In this study, we found that miR319 family members had similar sequences but different expression patterns in <i>Brassica campestris</i> and <i>Arabidopsis thaliana.</i> RT-PCR analysis revealed that <i>Bra-MIR319a</i> and <i>Bra-MIR319c</i> had similar expression patterns and were widely expressed in plant development, whereas <i>Bra-MIR319b</i> could only be detected in stems. The overexpression of each <i>Bra-MIR319</i> family member in <i>Arabidopsis</i> could inhibit cell division and function in leaf and petal morphogenesis. Bra-miR319a formed a new regulatory relationship after whole genome triplication, and <i>Bra-MIR319a</i> overexpressing in <i>Arabidopsis</i> led to the degradation of pollen content and affected the formation of intine, thereby causing pollen abortion. Our results suggest that <i>Bra-MIR319</i> family members have functional similarity and difference in plant development.