Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats

Background: To investigate the antilithiatic effect of hydroalcoholic extract of Kabab Chini (Piper cubeba L.) fruit in male Sprague Dawley rats. Methods: Rats were divided into six groups of six each. Group I received regular rat food and drinking water ad libitum. Groups II to VI were administered...

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Main Authors: Humaira Bano, Nasreen Jahan, Shaikh Ajij Ahmed Makbul, B.N. Kumar, Sadique Husain, Atiya Sayed
Format: Article
Language:English
Published: Elsevier 2018-12-01
Series:Integrative Medicine Research
Online Access:http://www.sciencedirect.com/science/article/pii/S2213422018301148
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spelling doaj-f8c49f8aa2b94caf9b46d939976dd6692020-11-24T21:28:54ZengElsevierIntegrative Medicine Research2213-42202018-12-0174358365Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley ratsHumaira Bano0Nasreen Jahan1Shaikh Ajij Ahmed Makbul2B.N. Kumar3Sadique Husain4Atiya Sayed5Department of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, IndiaDepartment of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, India; Corresponding author at: Department of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Kottigepalya, Magadi Main Road, Bangalore 560091, IndiaDepartment of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, IndiaDepartment of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, IndiaDepartment of Ilmul Advia (Pharmacology), National Institute of Unani Medicine, Bangalore, IndiaDepartment of Obstetrics and Gynecology, National Institute of Unani Medicine, Bangalore, IndiaBackground: To investigate the antilithiatic effect of hydroalcoholic extract of Kabab Chini (Piper cubeba L.) fruit in male Sprague Dawley rats. Methods: Rats were divided into six groups of six each. Group I received regular rat food and drinking water ad libitum. Groups II to VI were administered with ethylene glycol (EG) 0.75% (V/V) and ammonium chloride (AC) 1% (W/V) in drinking water for 7 days to induce urolithiasis. From 8th day Group I received 1 mL of 5% gum acacia. Group IV was treated with Cystone; V and VI groups with the hydro-alcoholic extract of Piper cubeba L. Treatment was continued for further 14 days, thereafter animals sacrificed. While Group II animals were sacrificed just after 7 days treatment with EG and AC. Group III was left untreated until 14 days and sacrificed on 22nd day. Crystalluria was analyzed on 8th and 22nd day while, urinary calcium, phosphorus, creatinine, sodium and magnesium on 22nd day. Biochemistry and histopathological studies of kidney were also carried out. Results: Test groups showed significant reduction (p < 0.001) of crystals in urine. Serum creatinine and urea (p < 0.01) were also decreased significantly. Urine analysis showed significant increase in magnesium while calcium, sodium, chloride and phosphorus significantly decreased along with histopathological improvement in kidney tissue in treated groups. Conclusion: From the above results it can be concluded that hydroalcoholic extract of P. cubeba L. fruit has significant inhibitory effect in calcium oxalate urolithiasis. Keywords: Unani medicine, Urolithiasis, Piper cubeba, Cystone, CaOx crystalshttp://www.sciencedirect.com/science/article/pii/S2213422018301148
collection DOAJ
language English
format Article
sources DOAJ
author Humaira Bano
Nasreen Jahan
Shaikh Ajij Ahmed Makbul
B.N. Kumar
Sadique Husain
Atiya Sayed
spellingShingle Humaira Bano
Nasreen Jahan
Shaikh Ajij Ahmed Makbul
B.N. Kumar
Sadique Husain
Atiya Sayed
Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
Integrative Medicine Research
author_facet Humaira Bano
Nasreen Jahan
Shaikh Ajij Ahmed Makbul
B.N. Kumar
Sadique Husain
Atiya Sayed
author_sort Humaira Bano
title Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
title_short Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
title_full Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
title_fullStr Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
title_full_unstemmed Effect of Piper cubeba L. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male Sprague Dawley rats
title_sort effect of piper cubeba l. fruit on ethylene glycol and ammonium chloride induced urolithiasis in male sprague dawley rats
publisher Elsevier
series Integrative Medicine Research
issn 2213-4220
publishDate 2018-12-01
description Background: To investigate the antilithiatic effect of hydroalcoholic extract of Kabab Chini (Piper cubeba L.) fruit in male Sprague Dawley rats. Methods: Rats were divided into six groups of six each. Group I received regular rat food and drinking water ad libitum. Groups II to VI were administered with ethylene glycol (EG) 0.75% (V/V) and ammonium chloride (AC) 1% (W/V) in drinking water for 7 days to induce urolithiasis. From 8th day Group I received 1 mL of 5% gum acacia. Group IV was treated with Cystone; V and VI groups with the hydro-alcoholic extract of Piper cubeba L. Treatment was continued for further 14 days, thereafter animals sacrificed. While Group II animals were sacrificed just after 7 days treatment with EG and AC. Group III was left untreated until 14 days and sacrificed on 22nd day. Crystalluria was analyzed on 8th and 22nd day while, urinary calcium, phosphorus, creatinine, sodium and magnesium on 22nd day. Biochemistry and histopathological studies of kidney were also carried out. Results: Test groups showed significant reduction (p < 0.001) of crystals in urine. Serum creatinine and urea (p < 0.01) were also decreased significantly. Urine analysis showed significant increase in magnesium while calcium, sodium, chloride and phosphorus significantly decreased along with histopathological improvement in kidney tissue in treated groups. Conclusion: From the above results it can be concluded that hydroalcoholic extract of P. cubeba L. fruit has significant inhibitory effect in calcium oxalate urolithiasis. Keywords: Unani medicine, Urolithiasis, Piper cubeba, Cystone, CaOx crystals
url http://www.sciencedirect.com/science/article/pii/S2213422018301148
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