Correlation between retention and C-18 silica gel surface coverage in reversed-phase liquid chromatography

• Main Authors: Ačanski Marijana M., Petrović Slobodan M., Pejanović Vjera M., Petrović Julijana A.
• Format: Article
• Language: English
• Published: Serbian Chemical Society 2000-01-01
• Series: Journal of the Serbian Chemical Society
• Subjects: hplc; reversed phase; estrone derivatives
• Online Access: http://www.doiserbia.nb.rs/img/doi/0352-5139/2000/0352-51390011811A.pdf
• ISSN: 0352-5139; 1820-7421

The effect of C-18 silica gel surface coverage on the retention behaviour of some estrone derivatives in reversed phase high performance liquid chromatography has been studied. Two commercial columns with different C-18 coverage, Spherisorb ODS-1(8 % carbon content) and Li Chrosorb RP-18(22% carbon content), using methanol-water as the eluent, were used.